BBa_K1369015 1 BBa_K1369015 fsrB_3xFLAG_N 2014-10-16T11:00:00Z 2015-05-08T01:10:08Z Enterococcus Faecalis Contains fsrB membrane protein from fsrABC quorum sensing system, just like BBa_K1369014, but adds 3xFLAG domain at the N-terminus (5' end) of the domain within fsrB responsible for coding GBAP (the signaling ligand in the fsrABC QS system). This serves as a target for antibody binding in Western blotting and purification columns. Available upon request from the Caltech 2014 iGEM team in a custom backbone. false false _1746_ 0 20035 9 Not in stock false Inserted 3xFLAG at N-terminus (5' end) of domain in fsrB encoding the prepeptide of the signaling ligand. Constructed by Gibson assembly. Not biobricked. false Galen Gao BBa_K1369015_sequence 1 atgctaatcgattggattctaaaaaatattatggatatggatcaggaagatcaatcaggaaaaacacaatggacaaagtattatctaaccgtttatttttctggcttatttaattttctgatgattctgattttatcagttttatttgggacgttaagcgaaacctttattgtatacgtcgtactgatttttttacggcctgtcgcaggtggctggcatgcaaaaactaaatggctctgtcgtctagaaagcattgttatctatgtcgccataccatttgtattgaaaaattcttctgtgagcttaccgtttatttataaaattctattgatttgcctcttagtcgtattattttattggtatgcgccacaaggaacagcaattgaacctgttcagccatctgatttaaacgtgctcaaaaagcaaagccttataagggtgtgtttacttattttatgtagtctgtttgtcaaagaaaagattgcttcagtaatactctacggtctcgtcatccaaggtctgatgatactccctgtaacaaaaaatttaattgaaggaagtgtttttatgaaatttggtaaaaaaataattaaaaatgttattgaaaaaagagttgcaaaagtcagtgatggtgtgggaactaagcctagattaaatgactacaaggaccatgacggtgattacaaggatcatgacatcgactacaaggatgacgatgacaagcaaaattcgcccaatatatttggacaatggatgggacaaactgaaaaacctaaaaagaatattgaaaaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z