BBa_K137013
1
BBa_K137013
folC (folylpolyglutamate synthase + dihydrofolate synthase)
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
L.lactis genome
folC is a necessary gene in the L.lactis folate biosynthesis gene cluster. folC is a bifunctional gene which encodes both folylpolyglutamate synthase and dihydrofolate synthase. These enzymes are needed for the last steps of dihydrofolate production.
true
false
_187_
0
2987
9
Discontinued
false
We were unsure of whether or not the L.lactis RBS would work with E. coli and so we are going to try cloning the entire gene cluster with the RBSs as well as cloning out each gene separately and adding E.coli RBSs.
false
Victoria Hsiao
annotation1963846
1
EcoR1 site
range1963846
1
979
979
annotation1963845
1
EcoR1 site
range1963845
1
860
860
BBa_K137013_sequence
1
atgtctattgaacaagcattagaatggatacattcacgattaaaatttaatattcgcccagggctaaatcgagtttcagcacttttagaattattaggcaatccagaggaatctttatcaatgattcacattgctggaacaaatgggaaaggatcaactgttgcctttacgcgctctatttttatgcaagctggcttaaaagttgcttcttttacgagtccttttattacgacttttggtgaacgaatgtctataaatgctcaaccaattcctgatgaaaagttaatttattatgtaaagatgattcaacctttagttgagcagcttgataatgatgctgagttgactggaattactgaatttgaaattattaccgcgatggcttttaaatattttgctgatgaaaaggttgatttggcggtaatcgaggttggcttgggtggactgcttgattcgacaaatgtcattagacccgttgtttctggaatcacaacaatcgggctagaccatattgatatcttgggttcaacgattgaagaaatcgcagctcaaaaagcaggtattattaaagctggaatacctgttgtggttggaaacattgaattaaaagctttgcgagttatttgggaagttgccagaaaaaatactgcgcgtatttatcagtatccttatgattaccgcaccgagttaaaagagcatgaacattttgatttctttgcaggtcaagaggcaattctggatttggaaaaatctttgattggcttgcatcaagtagaaaatgctggaatggccattgaattggcgctcgtctatgcgaataaagttggaattaacttgaatgaagagattattcgtgcgggaattcgtgaagctttttggcctgctcgaatggaaaaattaggcgaaaatcctttaattttacttgatggggctcataatgttcatgctatgaatcgtttgcttgagaacttggggaaagaattccctgataaaaaaattacaattattttttcagctattaccacaaaagatattaggcagatgataaaaatgcttcaaacggtaaaaaattctcgcttaattttgacaacttttgattatcctaaagctttagacttgaaagattttcggtatttagaggatgaaggggttgaattagcaccaagttgggaattagcgattgttcgtgcacaaaaagctttagatgaagatgagctcctattaatcactggttcgctctatttttcatcacaagttcgtgaatttttgaaaaaagaaaagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z