BBa_K137017 1 BBa_K137017 Galactose Oxidase 2008-07-14T11:00:00Z 2015-05-08T01:10:08Z Plasmid from the Arnold lab at CalTech, which is the result of their directed evolution to make galactose functionally expressed in E. coli and to improve its thermostability. Galactose oxidase is a fungal enzyme that uses molecular oxygen to oxidizes galactose and several other primary alcohols to the corresponding aldehyde, while also producing hydrogen peroxide. This particular galaxtose oxidase is not wild type. It has been optimized through directed evolution by the Arnold lab at CalTech to be functionally expressed in vivo in E. coli and to be thermostable. (Sun L, Petrounia IP, Yagasaki M, Bandara G, and Arnold FH. Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 2001 Sep; 14(9) 699-704. pmid:11707617). This parts has undergone two rounds of site directed mutagenesis to remove one EcoRI and one PstI site. In our hands, it is functional towards galactose in vivo. false false _187_ 0 3049 9 It's complicated false Two rounds of site directed mutagenesis had to be performed to eliminate an EcoRI and PstI site. false Doug Tischer annotation1967110 1 A->G range1967110 1 1386 1386 annotation1967109 1 T->C range1967109 1 522 522 BBa_K137017_sequence 1 atggcctcagcacctatcggaagcgccattcctcgcaacaactgggccgtcacttgcgacagtgcacagtcgggaaatgaatgcaacaaggccattgatggcaacaaggataccttttggcacacattctatggcgccaacggggatccaaagccccctcacacatacacgattgacatgaagacaactcagaacgtcaacggcttgtctgtgctgcctcgacaggatggtaaccaaaacggctggatcggtcgccatgaggtttatctaagctcagatggcacaaactggggcagccctgttgcgtcaggtagttggttcgccgactctactacaaaatactccaactttgaaactcgccctgctcgctatgttcgtcttgtcgctatcactgaagcgaatggccagccctggactagcattgcagagatcaacgtcttccaagctagttcttacacagccccccagcctggtcttggacgctggggtccgactattgacttaccgattgttcctgcggccgcagcaattgaaccgacatcgggacgagtccttatgtggtcttcatatcgcaatgatgcatttgaaggatcccctggtggtatcactttgacgtcttcctgggatccatccactggtattgtttccgaccgcactgtgacagtcaccaagcatgatatgttctgccctggtatctccatggatggtaacggtcagatcgtagtcacaggtggcaacgatgccaagaagaccagtttgtatgattcatctagcgatagctggatcccgggacctgacatgcaagtggctcgtgggtatcagtcatcagctaccatgtcagacggtcgtgtttttaccattggaggctcctggagcggtggcgtatttgagaagaatggcgaagtctatagcccatcttcaaagacatggacgtccctacccaatgccaaggtcaacccaatgttgacggctgacaagcaaggattgtaccgttcagacaaccacgcgtggctctttggatggaagaagggttcggtgttccaagcgggacctagcacagccatgaactggtactataccagtggaagtggtgatgtgaagtcagccggaaaacgccagtctaaccgtggtgtagcccctgatgccatgtgcggaaacgctgtcatgtacgacgccgttaaaggaaagatcctgacctttggcggctccccagattatcaagactctgacgccacaaccaacgcccacatcatcaccctcggtgaacccggaacatctcccaacactgtctttgctagcaatgggttgtactttgcccgaacgtttcacacctctgttgttcttccagacggaagcacgtttattacaggaggccaacgacgtgggattccgttcgaggattcaaccccggtatttacacctgagatctacgtccctgaacaagacactttctacaagcagaaccccaactccattgttcgcgcctaccatagcatttcccttttgttacctgatggcagggtatttaacggtggtggtggtctttgtggcgattgtaccacgaatcatttcgacgcgcaaatctttacgccaaactatctttacgatagcaacggcaatctcgcgacacgtcccaagattaccagaacctctacacagagcgtcaaggtcggtggcagaattacaatctcgacggattcttcgattagcaaggcgtcgttgattcgctatggtacagcgacacacacggttaatactgaccagcgccgcattcccctgactctgacaaacaatggaggaaatagctattctttccaagttcctagcgactctggtgttgctttgcctggctactggatgttgttcgtgatgaactcggccggtgttcctagtgtggcttcgacgattcgcgttactcagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z