BBa_K137025 1 BBa_K137025 GFP with (CTTCT)9 repeat after start codon 2008-07-19T11:00:00Z 2015-05-08T01:10:09Z The nucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run. GFP with (CTTCT)9 repeat after start codon false false _187_ 0 3112 9 Not in stock false With 9 CTTCT repeats, the GFP sequence is in frame with respect to the start codon, so a functional GFP should be transcribed. The number of CTTCT repeats was limited by maximum length of synthesized primers. false Allen Lin annotation1967759 1 CTTCT repeat range1967759 1 4 48 annotation1967758 1 ATG range1967758 1 1 1 BBa_K137025_sequence 1 atgcttctcttctcttctcttctcttctcttctcttctcttctcttctcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z