BBa_K137027
1
BBa_K137027
GFP with (AGTC)10 repeat after start codon
2008-07-19T11:00:00Z
2015-05-08T01:10:09Z
The tetranucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run.
GFP with (AGTC)10 repeat after start codon
false
false
_187_
0
3112
9
Not in stock
false
With 10 AGTC repeats, the GFP sequence is in the +1 frame with respect to the start codon, so GFP should not be transcribed.
The number of AGTC repeats was limited by maximum length of synthesized primers.
false
Allen Lin
annotation1967761
1
AGTC repeat
range1967761
1
5
43
annotation1967760
1
ATG
range1967760
1
1
1
BBa_K137027_sequence
1
atgagtcagtcagtcagtcagtcagtcagtcagtcagtcagtccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z