BBa_K137027 1 BBa_K137027 GFP with (AGTC)10 repeat after start codon 2008-07-19T11:00:00Z 2015-05-08T01:10:09Z The tetranucleotide repeat was placed into synthesized primers that bound to the existing part I13401, and then a PCR reaction was run. GFP with (AGTC)10 repeat after start codon false false _187_ 0 3112 9 Not in stock false With 10 AGTC repeats, the GFP sequence is in the +1 frame with respect to the start codon, so GFP should not be transcribed. The number of AGTC repeats was limited by maximum length of synthesized primers. false Allen Lin annotation1967761 1 AGTC repeat range1967761 1 5 43 annotation1967760 1 ATG range1967760 1 1 1 BBa_K137027_sequence 1 atgagtcagtcagtcagtcagtcagtcagtcagtcagtcagtccgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z