LOCUS       BBa_K137054             1251 bp    DNA     linear       26-Dec-2024
DEFINITION  Constitutive folKE expression construct
ACCESSION   BBa_K137054
VERSION     BBa_K137054.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1114
                     /label=BBa_K137011
     stem_loop       1134..1177
                     /label=stem_loop
     misc_feature    1123..1202
                     /label=BBa_B0010
     terminator      1123..1202
                     /label=BBa_B0010
     stem_loop       1218..1237
                     /label=T7 TE
     misc_feature    1244..1244
                     /label=stop
     polyA_site      1238..1251
                     /label=polya
     misc_feature    1211..1251
                     /label=BBa_B0012
     terminator      1211..1251
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgcaaaca acttatttaa gcatgggaag taatattggt gaccgtcagt attatttaca
      121 tgaagccatt cgtttattgg gaaaacaccc taaaattatg attgaaaaag tatcaaattt
      181 ttatgaaagt actccagtcg gcggcgtcaa acaagatgat tttactaatt tggcattaaa
      241 ggtggcaacg ctacttgaac ctttggaatt attatctttt attcatgaag ttgagctatc
      301 tttgaaccgt gagcgaaaaa ttcattgggg gccaagaaca attgatattg atattatttt
      361 ctataacgac ttagaaatgc aagaagaaaa cttggttatt ccacataaag aagcttttaa
      421 tcgtcttttt gtcttgaaac ctatttttga acttattgat aaagacttta aatattatgc
      481 gtcaatagaa aaagcaatag ccgaactttc agtaagtgaa caagagctcc atgtgataaa
      541 agaagaaaaa acaccaagaa atcgtattga agatgctgtt aaagagattc tctttgcagt
      601 aggtgaaaat ccaaatcgag aaggattact tgaaactcca gcgagagtag ctaaaatgta
      661 tgaagaaatt ctttcgtcac aacgcttaag caagtttaat gagtataaac tttttgaaat
      721 tgattcttct aaaacggatt caatcgtgtt gattaaagat attccttttt attcaatgtg
      781 tgagcatcat atgttaccat tttttgggaa agctcatgtt gcctatattc cagctgatgg
      841 aaaaattatt ggcttgtcaa aaattccccg tttagttgat tatgtttcgc gcaaactctc
      901 agttcaagaa aatatcactc atgatattgg agatgttttg actgatattt tgaatcctaa
      961 aggagtggca gttcttgttg aaggacgtca tatgtgcgtt gaaatgcgtg gagtaaaaaa
     1021 agtaaattct attactaaaa cttcttattt tttaggtgaa tttaaagaaa ataatgaaaa
     1081 aagaatggaa tttttagaaa gtcttttata ataatactag agccaggcat caaataaaac
     1141 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1201 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//