BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K137055 1 BBa_K137055 Constitutive pabA expression construct 2008-07-27T11:00:00Z 2015-05-08T01:10:09Z pabA has been extracted from the genome of L.lactis subspe. IL1403. Released HQ 2013 PabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter j23100 and the strong RBS b0034, and in front of the terminator b0015. Currently it is in the inducible copy plasmid psb2k3, which can be induced to high copy via IPTG. false false _187_ 0 2987 9 In stock true The purpose of this construct is to test the effects of pabA overexpression on the total production of pABA from chorismate. pABA is a necessary structural component in the synthesis of folate and so the hope is that by increasing the flux through the pABA synthesis pathway, we will be able to increase the total production of folate. true Victoria Hsiao component1969113 1 BBa_B0034 component1969117 1 BBa_B0012 component1969115 1 BBa_B0010 component1969114 1 BBa_K137005 component1969111 1 BBa_J23100 annotation1969117 1 BBa_B0012 range1969117 1 743 783 annotation1969111 1 BBa_J23100 range1969111 1 1 35 annotation1969113 1 BBa_B0034 range1969113 1 44 55 annotation1969114 1 BBa_K137005 range1969114 1 62 646 annotation1969115 1 BBa_B0010 range1969115 1 655 734 BBa_K137005 1 pabA pabA (from pABA synthesis) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome pabA is a gene involved in para-aminobenzoic acid (pABA) synthesis from chorismate. PABA is necessary for the synthesis of folate. false false _187_ 0 2987 9 It's complicated false We decided to clone the pABA synthesis genes separately (even though they reside side by side) because L.lactis uses start codons (ttg) that E.coli cannot, and so we need to change the start codon for pabB to atg. false Victoria Hsiao BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K137055_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_K137005_sequence 1 atgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z