BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K137055
1
BBa_K137055
Constitutive pabA expression construct
2008-07-27T11:00:00Z
2015-05-08T01:10:09Z
pabA has been extracted from the genome of L.lactis subspe. IL1403.
Released HQ 2013
PabA is one of three genes (pabA, pabB, pabC) involved in the para-aminobenzoic acid (pABA) synthesis pathway from chorismate. In this construct, pabA (para-aminobenzoate synthetase component II) has been placed behind the strong promoter j23100 and the strong RBS b0034, and in front of the terminator b0015. Currently it is in the inducible copy plasmid psb2k3, which can be induced to high copy via IPTG.
false
false
_187_
0
2987
9
In stock
true
The purpose of this construct is to test the effects of pabA overexpression on the total production of pABA from chorismate. pABA is a necessary structural component in the synthesis of folate and so the hope is that by increasing the flux through the pABA synthesis pathway, we will be able to increase the total production of folate.
true
Victoria Hsiao
component1969113
1
BBa_B0034
component1969117
1
BBa_B0012
component1969115
1
BBa_B0010
component1969114
1
BBa_K137005
component1969111
1
BBa_J23100
annotation1969117
1
BBa_B0012
range1969117
1
743
783
annotation1969111
1
BBa_J23100
range1969111
1
1
35
annotation1969113
1
BBa_B0034
range1969113
1
44
55
annotation1969114
1
BBa_K137005
range1969114
1
62
646
annotation1969115
1
BBa_B0010
range1969115
1
655
734
BBa_K137005
1
pabA
pabA (from pABA synthesis)
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
L.lactis genome
pabA is a gene involved in para-aminobenzoic acid (pABA) synthesis from chorismate. PABA is necessary for the synthesis of folate.
false
false
_187_
0
2987
9
It's complicated
false
We decided to clone the pABA synthesis genes separately (even though they reside side by side) because L.lactis uses start codons (ttg) that E.coli cannot, and so we need to change the start codon for pabB to atg.
false
Victoria Hsiao
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K137055_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K137005_sequence
1
atgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z