LOCUS       BBa_K137057             1119 bp    DNA     linear       23-May-2024
DEFINITION  GFP fimE switch with 150 bp tetR promoter
ACCESSION   BBa_K137057
VERSION     BBa_K137057.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRL in
     misc_feature    19..35
                     /label=IRL out
     misc_feature    1..35
                     /label=BBa_K137010
     promoter        44..97
                     /label=tetR promoter
     promoter        44..193
                     /label=BBa_K137046
     misc_feature    202..218
                     /label=IRR out
     misc_feature    220..236
                     /label=IRR in
     misc_feature    202..236
                     /label=BBa_K137008
     misc_feature    249..252
                     /label=conserved
     RBS             245..256
                     /label=BBa_B0034
     CDS             263..982
                     /label=GFP protein
     CDS             263..982
                     /label=BBa_E0040
     stem_loop       1002..1045
                     /label=stem_loop
     misc_feature    991..1070
                     /label=BBa_B0010
     terminator      991..1070
                     /label=BBa_B0010
     stem_loop       1086..1105
                     /label=T7 TE
     misc_feature    1112..1112
                     /label=stop
     polyA_site      1106..1119
                     /label=polya
     misc_feature    1079..1119
                     /label=BBa_B0012
     terminator      1079..1119
                     /label=BBa_B0012
ORIGIN
        1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggctactaga gaagatgaaa catttggggc caaactgtcc atattatact
      241 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      301 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      361 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      421 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      481 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      541 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      601 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      661 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      721 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      781 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      841 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
      901 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
      961 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1021 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1081 acactggctc accttcgggt gggcctttct gcgtttata
//