LOCUS       BBa_K137060             1419 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP fimE switch with 450 bp tetR promoter
ACCESSION   BBa_K137060
VERSION     BBa_K137060.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRL in
     misc_feature    19..35
                     /label=IRL out
     misc_feature    1..35
                     /label=BBa_K137010
     promoter        44..97
                     /label=tetR promotoer
     promoter        44..493
                     /label=BBa_K137049
     misc_feature    502..518
                     /label=IRR out
     misc_feature    520..536
                     /label=IRR in
     misc_feature    502..536
                     /label=BBa_K137008
     misc_feature    549..552
                     /label=conserved
     RBS             545..556
                     /label=BBa_B0034
     CDS             563..1282
                     /label=GFP protein
     CDS             563..1282
                     /label=BBa_E0040
     stem_loop       1302..1345
                     /label=stem_loop
     misc_feature    1291..1370
                     /label=BBa_B0010
     terminator      1291..1370
                     /label=BBa_B0010
     stem_loop       1386..1405
                     /label=T7 TE
     misc_feature    1412..1412
                     /label=stop
     polyA_site      1406..1419
                     /label=polya
     misc_feature    1379..1419
                     /label=BBa_B0012
     terminator      1379..1419
                     /label=BBa_B0012
ORIGIN
        1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
      301 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      481 ttgatcttcc aattactaga gaagatgaaa catttggggc caaactgtcc atattatact
      541 agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
      601 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      661 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      721 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      781 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      841 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      901 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      961 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     1021 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     1081 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     1141 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     1201 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     1261 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     1321 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1381 acactggctc accttcgggt gggcctttct gcgtttata
//