LOCUS BBa_K137061 1619 bp DNA linear 22-Nov-2024 DEFINITION GFP fimE switch with 650 bp tetR promoter ACCESSION BBa_K137061 VERSION BBa_K137061.1 FEATURES Location/Qualifiers misc_feature 1..17 /label=IRL in misc_feature 19..35 /label=IRL out misc_feature 1..35 /label=BBa_K137010 promoter 44..97 /label=tetR promoter promoter 44..693 /label=BBa_K137050 misc_feature 702..718 /label=IRR out misc_feature 720..736 /label=IRR in misc_feature 702..736 /label=BBa_K137008 misc_feature 749..752 /label=conserved RBS 745..756 /label=BBa_B0034 CDS 763..1482 /label=GFP protein CDS 763..1482 /label=BBa_E0040 stem_loop 1502..1545 /label=stem_loop misc_feature 1491..1570 /label=BBa_B0010 terminator 1491..1570 /label=BBa_B0010 stem_loop 1586..1605 /label=T7 TE misc_feature 1612..1612 /label=stop polyA_site 1606..1619 /label=polya misc_feature 1579..1619 /label=BBa_B0012 terminator 1579..1619 /label=BBa_B0012 ORIGIN 1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat 61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag 121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga 181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag 241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt 301 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag 361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa 421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc 481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc 541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata 601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa 661 agcacatcta aaacttttag cgttattacg taatactaga gaagatgaaa catttggggc 721 caaactgtcc atattatact agagaaagag gagaaatact agatgcgtaa aggagaagaa 781 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 841 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 901 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 961 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 1021 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 1081 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 1141 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 1201 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 1261 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 1321 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 1381 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct 1441 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca 1501 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 1561 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //