LOCUS       BBa_K137061             1619 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP fimE switch with 650 bp tetR promoter
ACCESSION   BBa_K137061
VERSION     BBa_K137061.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRL in
     misc_feature    19..35
                     /label=IRL out
     misc_feature    1..35
                     /label=BBa_K137010
     promoter        44..97
                     /label=tetR promoter
     promoter        44..693
                     /label=BBa_K137050
     misc_feature    702..718
                     /label=IRR out
     misc_feature    720..736
                     /label=IRR in
     misc_feature    702..736
                     /label=BBa_K137008
     misc_feature    749..752
                     /label=conserved
     RBS             745..756
                     /label=BBa_B0034
     CDS             763..1482
                     /label=GFP protein
     CDS             763..1482
                     /label=BBa_E0040
     stem_loop       1502..1545
                     /label=stem_loop
     misc_feature    1491..1570
                     /label=BBa_B0010
     terminator      1491..1570
                     /label=BBa_B0010
     stem_loop       1586..1605
                     /label=T7 TE
     misc_feature    1612..1612
                     /label=stop
     polyA_site      1606..1619
                     /label=polya
     misc_feature    1579..1619
                     /label=BBa_B0012
     terminator      1579..1619
                     /label=BBa_B0012
ORIGIN
        1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
      301 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      661 agcacatcta aaacttttag cgttattacg taatactaga gaagatgaaa catttggggc
      721 caaactgtcc atattatact agagaaagag gagaaatact agatgcgtaa aggagaagaa
      781 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
      841 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
      901 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
      961 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     1021 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     1081 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     1141 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     1201 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     1261 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     1321 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1381 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     1441 gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca
     1501 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1561 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//