LOCUS       BBa_K137062             1819 bp    DNA     linear       26-Nov-2024
DEFINITION  GFP fimE switch with 850 bp tetR promoter
ACCESSION   BBa_K137062
VERSION     BBa_K137062.1  
FEATURES             Location/Qualifiers
     misc_feature    1..17
                     /label=IRL in
     misc_feature    19..35
                     /label=IRL out
     misc_feature    1..35
                     /label=BBa_K137010
     promoter        44..97
                     /label=tetR promoter
     promoter        44..893
                     /label=BBa_K137051
     misc_feature    902..918
                     /label=IRR out
     misc_feature    920..936
                     /label=IRR in
     misc_feature    902..936
                     /label=BBa_K137008
     misc_feature    949..952
                     /label=conserved
     RBS             945..956
                     /label=BBa_B0034
     CDS             963..1682
                     /label=GFP protein
     CDS             963..1682
                     /label=BBa_E0040
     stem_loop       1702..1745
                     /label=stem_loop
     misc_feature    1691..1770
                     /label=BBa_B0010
     terminator      1691..1770
                     /label=BBa_B0010
     stem_loop       1786..1805
                     /label=T7 TE
     misc_feature    1812..1812
                     /label=stop
     polyA_site      1806..1819
                     /label=polya
     misc_feature    1779..1819
                     /label=BBa_B0012
     terminator      1779..1819
                     /label=BBa_B0012
ORIGIN
        1 tctatgagtc aaaatggccc caattgtctt gtatttacta gaggtgctca gtatctctat
       61 cactgatagg gatgtcaatc tctatcactg atagggactc tagtatataa acgcagaaag
      121 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      181 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      241 tagtgatcta cactagcact atcagtgtta ttaagctact aaagcgtagt tttcgtcgtt
      301 tgcagcggac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
      361 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
      421 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
      481 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
      541 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
      601 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
      661 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
      721 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
      781 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
      841 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctactaga
      901 gaagatgaaa catttggggc caaactgtcc atattatact agagaaagag gagaaatact
      961 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     1021 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     1081 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     1141 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     1201 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     1261 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     1321 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     1381 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     1441 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1501 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1561 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1621 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1681 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1801 gggcctttct gcgtttata
//