LOCUS       BBa_K137063             3277 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter with (TA)10 repeat in front of RBS-LacZ-TT
ACCESSION   BBa_K137063
VERSION     BBa_K137063.1  
FEATURES             Location/Qualifiers
     misc_feature    23..28
                     /label=-10 element
     misc_feature    7..22
                     /label=TA repeat
     promoter        1..39
                     /label=BBa_K137029
     misc_feature    52..55
                     /label=conserved
     RBS             48..59
                     /label=BBa_B0034
     misc_feature    3089..3089
                     /label=C->T
     CDS             66..3140
                     /label=lacZ
     misc_feature    66..68
                     /label=START
     misc_feature    3138..3140
                     /label=STOP
     CDS             66..3140
                     /label=BBa_I732005
     stem_loop       3160..3203
                     /label=stem_loop
     misc_feature    3149..3228
                     /label=BBa_B0010
     terminator      3149..3228
                     /label=BBa_B0010
     stem_loop       3244..3263
                     /label=T7 TE
     misc_feature    3270..3270
                     /label=stop
     polyA_site      3264..3277
                     /label=polya
     misc_feature    3237..3277
                     /label=BBa_B0012
     terminator      3237..3277
                     /label=BBa_B0012
ORIGIN
        1 tttaattata tatatatata tatataatgg aagcgttttt actagagaaa gaggagaaat
       61 actagatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      121 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      181 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      241 ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
      301 ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
      361 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
      421 atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
      481 agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
      541 gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
      601 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
      661 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
      721 cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg
      781 ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag
      841 tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
      901 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
      961 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
     1021 ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg
     1081 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg
     1141 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
     1201 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
     1261 ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc
     1321 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
     1381 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
     1441 tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg
     1501 tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
     1561 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat
     1621 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
     1681 acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc
     1741 agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
     1801 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
     1861 atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga
     1921 cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
     1981 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
     2041 tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
     2101 agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag
     2161 tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc
     2221 agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc
     2281 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
     2341 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
     2401 cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag
     2461 cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg
     2521 ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga
     2581 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
     2641 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc
     2701 atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
     2761 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
     2821 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
     2881 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
     2941 acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
     3001 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact
     3061 cctggagccc gtcagtatcg gcggaatttc agctgagcgc cggtcgctac cattaccagt
     3121 tggtctggtg tcaaaaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3181 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3241 actggctcac cttcgggtgg gcctttctgc gtttata
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