LOCUS       BBa_K137064             3275 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter with (TA)9 repeat in front of RBS-LacZ-TT
ACCESSION   BBa_K137064
VERSION     BBa_K137064.1  
FEATURES             Location/Qualifiers
     misc_feature    21..25
                     /label=-10 element
     misc_feature    7..20
                     /label=TA repeat
     promoter        1..37
                     /label=BBa_K137030
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    3087..3087
                     /label=C->T
     CDS             64..3138
                     /label=lacZ
     misc_feature    64..66
                     /label=START
     misc_feature    3136..3138
                     /label=STOP
     CDS             64..3138
                     /label=BBa_I732005
     stem_loop       3158..3201
                     /label=stem_loop
     misc_feature    3147..3226
                     /label=BBa_B0010
     terminator      3147..3226
                     /label=BBa_B0010
     stem_loop       3242..3261
                     /label=T7 TE
     misc_feature    3268..3268
                     /label=stop
     polyA_site      3262..3275
                     /label=polya
     misc_feature    3235..3275
                     /label=BBa_B0012
     terminator      3235..3275
                     /label=BBa_B0012
ORIGIN
        1 tttaattata tatatatata tataatggaa gcgtttttac tagagaaaga ggagaaatac
       61 tagatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      121 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      181 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      241 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
      301 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
      361 atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc cacggagaat
      421 ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca ggaaggccag
      481 acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
      541 gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc atttttacgc
      601 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
      661 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcataaaccg
      721 actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt cagccgcgct
      781 gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg ggtaacagtt
      841 tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
      901 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
      961 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
     1021 gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa
     1081 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag
     1141 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
     1201 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
     1261 tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat tgaaacccac
     1321 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
     1381 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
     1441 gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat caaatctgtc
     1501 gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
     1561 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
     1621 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
     1681 gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc gtttcgtcag
     1741 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
     1801 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
     1861 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
     1921 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
     1981 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
     2041 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
     2101 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
     2161 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag cgcctggcag
     2221 cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca cgccatcccg
     2281 catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg ttggcaattt
     2341 aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca actgctgacg
     2401 ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
     2461 acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
     2521 gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct gattacgacc
     2581 gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
     2641 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga tacaccgcat
     2701 ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt aaactggctc
     2761 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
     2821 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
     2881 tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt ccagttcaac
     2941 atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct gctgcacgcg
     3001 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
     3061 tggagcccgt cagtatcggc ggaatttcag ctgagcgccg gtcgctacca ttaccagttg
     3121 gtctggtgtc aaaaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     3181 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     3241 tggctcacct tcgggtgggc ctttctgcgt ttata
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