LOCUS       BBa_K137065             3300 bp    DNA     linear       14-May-2024
DEFINITION  Promoter with (C)10 repeat in front of RBS-LacZ-TT
ACCESSION   BBa_K137065
VERSION     BBa_K137065.1  
FEATURES             Location/Qualifiers
     misc_feature    50..55
                     /label=-10
     misc_feature    27..36
                     /label=C repeat
     promoter        1..62
                     /label=BBa_K137031
     misc_feature    75..78
                     /label=conserved
     RBS             71..82
                     /label=BBa_B0034
     misc_feature    3112..3112
                     /label=C->T
     CDS             89..3163
                     /label=lacZ
     misc_feature    89..91
                     /label=START
     misc_feature    3161..3163
                     /label=STOP
     CDS             89..3163
                     /label=BBa_I732005
     stem_loop       3183..3226
                     /label=stem_loop
     misc_feature    3172..3251
                     /label=BBa_B0010
     terminator      3172..3251
                     /label=BBa_B0010
     stem_loop       3267..3286
                     /label=T7 TE
     misc_feature    3293..3293
                     /label=stop
     polyA_site      3287..3300
                     /label=polya
     misc_feature    3260..3300
                     /label=BBa_B0012
     terminator      3260..3300
                     /label=BBa_B0012
ORIGIN
        1 ttaattttta ttaatatatg taaaatcccc ccccccgaaa gcttaagaat ataattgtaa
       61 gctactagag aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc
      301 ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg
      361 gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa
      421 tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga
      481 tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt
      541 tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga
      601 atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg
      661 ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg
      721 tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg
      781 ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt
      841 gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg
      901 caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac
      961 actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg
     1021 tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt
     1081 cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct
     1141 gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca
     1201 gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc
     1261 gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt
     1321 ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc
     1381 gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca
     1441 cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc
     1501 gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg
     1561 agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca
     1621 gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac
     1681 gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc
     1741 taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg
     1801 ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg
     1861 tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga
     1921 ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg
     1981 tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga
     2041 gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct
     2101 ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag
     2161 cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga
     2221 agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct
     2281 ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga
     2341 gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat
     2401 tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga
     2461 taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg
     2521 gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact
     2581 tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt
     2641 tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt
     2701 tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca
     2761 ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct
     2821 tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt
     2881 cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca
     2941 gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac
     3001 cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca
     3061 tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag
     3121 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taatactaga gccaggcatc
     3181 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     3241 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//