LOCUS       BBa_K137066             3302 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter with (C)12 repeat in front of RBS-LacZ-TT
ACCESSION   BBa_K137066
VERSION     BBa_K137066.1  
FEATURES             Location/Qualifiers
     misc_feature    52..57
                     /label=-10 element
     misc_feature    27..38
                     /label=c repeat
     promoter        1..64
                     /label=BBa_K137032
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    3114..3114
                     /label=C->T
     CDS             91..3165
                     /label=lacZ
     misc_feature    91..93
                     /label=START
     misc_feature    3163..3165
                     /label=STOP
     CDS             91..3165
                     /label=BBa_I732005
     stem_loop       3185..3228
                     /label=stem_loop
     misc_feature    3174..3253
                     /label=BBa_B0010
     terminator      3174..3253
                     /label=BBa_B0010
     stem_loop       3269..3288
                     /label=T7 TE
     misc_feature    3295..3295
                     /label=stop
     polyA_site      3289..3302
                     /label=polya
     misc_feature    3262..3302
                     /label=BBa_B0012
     terminator      3262..3302
                     /label=BBa_B0012
ORIGIN
        1 ttaattttta ttaatatatg taaaatcccc ccccccccga aagcttaaga atataattgt
       61 aagctactag agaaagagga gaaatactag atgaccatga ttacggattc actggccgtc
      121 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      181 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      241 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      301 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
      361 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
      421 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
      481 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
      541 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
      601 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
      661 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
      721 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
      781 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
      841 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
      901 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
      961 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
     1021 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     1081 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     1141 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     1201 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     1261 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     1321 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     1381 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     1441 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     1501 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     1561 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     1621 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     1681 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     1741 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     1801 tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc
     1861 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     1921 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     1981 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     2041 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     2101 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     2161 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     2221 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     2281 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     2341 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     2401 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     2461 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     2521 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     2581 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     2641 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     2701 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     2761 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     2821 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     2881 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     2941 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     3001 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     3061 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga atttcagctg
     3121 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataatacta gagccaggca
     3181 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     3241 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     3301 ta
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