LOCUS BBa_K137076 3004 bp DNA linear 25-Nov-2024 DEFINITION AHL inducible production of hydrogen peroxide ACCESSION BBa_K137076 VERSION BBa_K137076.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1115..1115 /label=T->G CDS 1089..2867 /label=BBa_K137069 stem_loop 2887..2930 /label=stem_loop misc_feature 2876..2955 /label=BBa_B0010 terminator 2876..2955 /label=BBa_B0010 stem_loop 2971..2990 /label=T7 TE misc_feature 2997..2997 /label=stop polyA_site 2991..3004 /label=polya misc_feature 2964..3004 /label=BBa_B0012 terminator 2964..3004 /label=BBa_B0012 terminator 2876..3004 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gactcaaggg aaaattactg catcggcagc aatgcttaac gtattgaaaa 1141 catggggcgt agatacaatc tacggtatcc catcaggaac actcagctca ttgatggacg 1201 ctttggctga agacaaagat atccgcttct tacaagttcg ccacgaagag acaggtgctc 1261 ttgcagcggt tatgcaagct aaattcggcg gctcaatcgg ggttgcagtt ggttcaggtg 1321 gtccaggtgc gactcacttg attaacggtg tttacgatgc agctatggat gacactccat 1381 tcctagcgat ccttggatca cgtccagtta acgaattgaa catggatgct ttccaagagc 1441 ttaaccaaaa cccaatgtac aacggtatcg ctgtttacaa caaacgtgta gcttacgctg 1501 agcaattgcc aaaagtaatt gacgaagcct gccgtgctgc aatttctaaa aaaggtccag 1561 ctgttgttga aattccagta aacttcggtt tccaagaaat cgacgaaaac tcatactacg 1621 gttcaggttc atacgaacgc tcattcatcg ctcctgcttt gaacgaagtt gaaatcgaca 1681 aagctgttga aatcttgaac aatgctgaac gcccagttat ctatgctgga tttggtggtg 1741 ttaaagctgg tgaagtgatt actgaattgt cacgtaaaat caaagcacca atcatcacaa 1801 ctggtaaaaa ctttgaagct ttcgaatgga actatgaagg tttgacaggt tctgcttacc 1861 gtgttggttg gaaaccagcc aacgaagtgg tctttgaagc agacacagtt cttttccttg 1921 gttcaaactt cgcatttgct gaagtttacg aagcattcaa gaacactgaa aaattcatac 1981 aagtcgatat cgacccttac aaacttggta aacgtcatgc ccttgacgct tcaatccttg 2041 gtgatgctgg tcaagcagct aaagctatcc ttgacaaagt aaacccagtt gaatcaactc 2101 catggtggcg tgcaaacgtt aagaacaacc aaaactggcg tgattacatg aacaaactcg 2161 aaggtaaaac tgagggtgaa ttgcaattgt atcaagttta caatgcaatc aacaaacatg 2221 ctgatcaaga cgctatctac tcaatcgacg taggtaacac tactcaaaca tctactcgtc 2281 accttcacat gacacctaag aatatgtggc gtacatctcc gctctttgcg acaatgggta 2341 ttgcccttcc tggtggtatc gctgctaaga aagacaatcc agatcgccaa gtatggaaca 2401 tcatgggtga tggagcattc aacatgtgct acccagacgt tatcacaaac gttcaatacg 2461 accttccagt tatcaacctt gtcttctcaa atgctgagta cggcttcatc aagaacaaat 2521 acgaagatac aaacaaacac ttgtttggtg tagacttcac aaacgctgac tacgctaaaa 2581 ttgcggaagc tcaaggagct gttggattca cagttgaccg tatcgaagac atcgatgcag 2641 ttgttgcaga agctgttaaa ttgaacaaag aaggtaaaac tgttgtcatc gatgctcgca 2701 tcactcaaca ccgtccactt ccagtagaag tacttgaatt ggatccaaaa cttcactcag 2761 aagaagctat caaagccttc aaggaaaaat acgaagcaga agaactcgta ccattccgtc 2821 tcttcttgga agaagaagga ttgcaatcac gcgcaattaa ataataatac tagagccagg 2881 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2941 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3001 tata //