LOCUS       BBa_K137077             1977 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutively produces pyruvate oxidase.
ACCESSION   BBa_K137077
VERSION     BBa_K137077.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    88..88
                     /label=T->G
     CDS             62..1840
                     /label=BBa_K137069
     stem_loop       1860..1903
                     /label=stem_loop
     misc_feature    1849..1928
                     /label=BBa_B0010
     terminator      1849..1928
                     /label=BBa_B0010
     stem_loop       1944..1963
                     /label=T7 TE
     misc_feature    1970..1970
                     /label=stop
     polyA_site      1964..1977
                     /label=polya
     misc_feature    1937..1977
                     /label=BBa_B0012
     terminator      1937..1977
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgactcaa gggaaaatta ctgcatcggc agcaatgctt aacgtattga aaacatgggg
      121 cgtagataca atctacggta tcccatcagg aacactcagc tcattgatgg acgctttggc
      181 tgaagacaaa gatatccgct tcttacaagt tcgccacgaa gagacaggtg ctcttgcagc
      241 ggttatgcaa gctaaattcg gcggctcaat cggggttgca gttggttcag gtggtccagg
      301 tgcgactcac ttgattaacg gtgtttacga tgcagctatg gatgacactc cattcctagc
      361 gatccttgga tcacgtccag ttaacgaatt gaacatggat gctttccaag agcttaacca
      421 aaacccaatg tacaacggta tcgctgttta caacaaacgt gtagcttacg ctgagcaatt
      481 gccaaaagta attgacgaag cctgccgtgc tgcaatttct aaaaaaggtc cagctgttgt
      541 tgaaattcca gtaaacttcg gtttccaaga aatcgacgaa aactcatact acggttcagg
      601 ttcatacgaa cgctcattca tcgctcctgc tttgaacgaa gttgaaatcg acaaagctgt
      661 tgaaatcttg aacaatgctg aacgcccagt tatctatgct ggatttggtg gtgttaaagc
      721 tggtgaagtg attactgaat tgtcacgtaa aatcaaagca ccaatcatca caactggtaa
      781 aaactttgaa gctttcgaat ggaactatga aggtttgaca ggttctgctt accgtgttgg
      841 ttggaaacca gccaacgaag tggtctttga agcagacaca gttcttttcc ttggttcaaa
      901 cttcgcattt gctgaagttt acgaagcatt caagaacact gaaaaattca tacaagtcga
      961 tatcgaccct tacaaacttg gtaaacgtca tgcccttgac gcttcaatcc ttggtgatgc
     1021 tggtcaagca gctaaagcta tccttgacaa agtaaaccca gttgaatcaa ctccatggtg
     1081 gcgtgcaaac gttaagaaca accaaaactg gcgtgattac atgaacaaac tcgaaggtaa
     1141 aactgagggt gaattgcaat tgtatcaagt ttacaatgca atcaacaaac atgctgatca
     1201 agacgctatc tactcaatcg acgtaggtaa cactactcaa acatctactc gtcaccttca
     1261 catgacacct aagaatatgt ggcgtacatc tccgctcttt gcgacaatgg gtattgccct
     1321 tcctggtggt atcgctgcta agaaagacaa tccagatcgc caagtatgga acatcatggg
     1381 tgatggagca ttcaacatgt gctacccaga cgttatcaca aacgttcaat acgaccttcc
     1441 agttatcaac cttgtcttct caaatgctga gtacggcttc atcaagaaca aatacgaaga
     1501 tacaaacaaa cacttgtttg gtgtagactt cacaaacgct gactacgcta aaattgcgga
     1561 agctcaagga gctgttggat tcacagttga ccgtatcgaa gacatcgatg cagttgttgc
     1621 agaagctgtt aaattgaaca aagaaggtaa aactgttgtc atcgatgctc gcatcactca
     1681 acaccgtcca cttccagtag aagtacttga attggatcca aaacttcact cagaagaagc
     1741 tatcaaagcc ttcaaggaaa aatacgaagc agaagaactc gtaccattcc gtctcttctt
     1801 ggaagaagaa ggattgcaat cacgcgcaat taaataataa tactagagcc aggcatcaaa
     1861 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1921 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//