LOCUS BBa_K137078 6157 bp DNA linear 25-Nov-2024 DEFINITION AHL -> H2O2 ACCESSION BBa_K137078 VERSION BBa_K137078.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1..1082 /label=BBa_S03975 misc_feature 1115..1115 /label=T->G CDS 1089..2867 /label=BBa_K137069 misc_feature 1..2867 /label=BBa_S04051 misc_feature 2880..2883 /label=conserved RBS 2876..2887 /label=BBa_B0034 CDS 2897..3513 /label=tetR misc_feature 3514..3547 /label=SsrA misc_feature 3554..3578 /label=Help:Barcodes CDS 2894..3553 /label=BBa_C0040 stem_loop 3598..3641 /label=stem_loop misc_feature 3587..3666 /label=BBa_B0010 terminator 3587..3666 /label=BBa_B0010 stem_loop 3682..3701 /label=T7 TE misc_feature 3708..3708 /label=stop polyA_site 3702..3715 /label=polya misc_feature 3675..3715 /label=BBa_B0012 terminator 3675..3715 /label=BBa_B0012 misc_feature 3724..3742 /label=TetR 1 misc_feature 3724..3777 /label=BBa_R0040 promoter 3743..3748 /label=-35 misc_feature 3749..3767 /label=TetR 2 promoter 3766..3771 /label=-10 promoter 3724..3777 /label=BBa_R0040 misc_feature 2876..3777 /label=BBa_Q04400 misc_feature 3790..3793 /label=conserved RBS 3786..3797 /label=BBa_B0034 misc_feature 2876..3797 /label=BBa_S03948 misc_feature 1..3797 /label=BBa_S04057 misc_feature 4001..4001 /label=C->T CDS 3804..6020 /label=BBa_K137068 stem_loop 6040..6083 /label=stem_loop misc_feature 6029..6108 /label=BBa_B0010 terminator 6029..6108 /label=BBa_B0010 stem_loop 6124..6143 /label=T7 TE misc_feature 6150..6150 /label=stop polyA_site 6144..6157 /label=polya misc_feature 6117..6157 /label=BBa_B0012 terminator 6117..6157 /label=BBa_B0012 misc_feature 3804..6157 /label=BBa_S04059 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gactcaaggg aaaattactg catcggcagc aatgcttaac gtattgaaaa 1141 catggggcgt agatacaatc tacggtatcc catcaggaac actcagctca ttgatggacg 1201 ctttggctga agacaaagat atccgcttct tacaagttcg ccacgaagag acaggtgctc 1261 ttgcagcggt tatgcaagct aaattcggcg gctcaatcgg ggttgcagtt ggttcaggtg 1321 gtccaggtgc gactcacttg attaacggtg tttacgatgc agctatggat gacactccat 1381 tcctagcgat ccttggatca cgtccagtta acgaattgaa catggatgct ttccaagagc 1441 ttaaccaaaa cccaatgtac aacggtatcg ctgtttacaa caaacgtgta gcttacgctg 1501 agcaattgcc aaaagtaatt gacgaagcct gccgtgctgc aatttctaaa aaaggtccag 1561 ctgttgttga aattccagta aacttcggtt tccaagaaat cgacgaaaac tcatactacg 1621 gttcaggttc atacgaacgc tcattcatcg ctcctgcttt gaacgaagtt gaaatcgaca 1681 aagctgttga aatcttgaac aatgctgaac gcccagttat ctatgctgga tttggtggtg 1741 ttaaagctgg tgaagtgatt actgaattgt cacgtaaaat caaagcacca atcatcacaa 1801 ctggtaaaaa ctttgaagct ttcgaatgga actatgaagg tttgacaggt tctgcttacc 1861 gtgttggttg gaaaccagcc aacgaagtgg tctttgaagc agacacagtt cttttccttg 1921 gttcaaactt cgcatttgct gaagtttacg aagcattcaa gaacactgaa aaattcatac 1981 aagtcgatat cgacccttac aaacttggta aacgtcatgc ccttgacgct tcaatccttg 2041 gtgatgctgg tcaagcagct aaagctatcc ttgacaaagt aaacccagtt gaatcaactc 2101 catggtggcg tgcaaacgtt aagaacaacc aaaactggcg tgattacatg aacaaactcg 2161 aaggtaaaac tgagggtgaa ttgcaattgt atcaagttta caatgcaatc aacaaacatg 2221 ctgatcaaga cgctatctac tcaatcgacg taggtaacac tactcaaaca tctactcgtc 2281 accttcacat gacacctaag aatatgtggc gtacatctcc gctctttgcg acaatgggta 2341 ttgcccttcc tggtggtatc gctgctaaga aagacaatcc agatcgccaa gtatggaaca 2401 tcatgggtga tggagcattc aacatgtgct acccagacgt tatcacaaac gttcaatacg 2461 accttccagt tatcaacctt gtcttctcaa atgctgagta cggcttcatc aagaacaaat 2521 acgaagatac aaacaaacac ttgtttggtg tagacttcac aaacgctgac tacgctaaaa 2581 ttgcggaagc tcaaggagct gttggattca cagttgaccg tatcgaagac atcgatgcag 2641 ttgttgcaga agctgttaaa ttgaacaaag aaggtaaaac tgttgtcatc gatgctcgca 2701 tcactcaaca ccgtccactt ccagtagaag tacttgaatt ggatccaaaa cttcactcag 2761 aagaagctat caaagccttc aaggaaaaat acgaagcaga agaactcgta ccattccgtc 2821 tcttcttgga agaagaagga ttgcaatcac gcgcaattaa ataataatac tagagaaaga 2881 ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct 2941 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt 3001 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc 3061 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca 3121 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg 3181 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca 3241 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc 3301 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga 3361 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt 3421 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg 3481 attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt 3541 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 3601 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 3661 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 3721 gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac 3781 tagagaaaga ggagaaatac tagatgagca cgtcagacga tatccataac accacagcca 3841 ctggcaaatg cccgttccat cagggcggtc acgaccagag tgcgggggcg ggcacaacca 3901 ctcgcgactg gtggccaaat caacttcgtg ttgacctgtt aaaccaacat tctaatcgtt 3961 ctaacccact gggtgaggac tttgactacc gcaaagaatt tagcaaatta gattactacg 4021 gcctgaaaaa agatctgaaa gccctgttga cagaatctca accgtggtgg ccagccgact 4081 ggggcagtta cgccggtctg tttattcgta tggcctggca cggcgcgggg acttaccgtt 4141 caatcgatgg acgcggtggc gcgggtcgtg gtcagcaacg ttttgcaccg ctgaactcct 4201 ggccggataa cgtaagcctc gataaagcgc gtcgcctgtt gtggccaatc aaacagaaat 4261 atggtcagaa aatctcctgg gccgacctgt ttatcctcgc gggtaacgtg gcgctagaaa 4321 actccggctt ccgtaccttc ggttttggtg ccggtcgtga agacgtctgg gaaccggatc 4381 tggatgttaa ctggggtgat gaaaaagcct ggctgactca ccgtcatccg gaagcgctgg 4441 cgaaagcacc gctgggtgca accgagatgg gtctgattta cgttaacccg gaaggcccgg 4501 atcacagcgg cgaaccgctt tctgcggcag cagctatccg cgcgaccttc ggcaacatgg 4561 gcatgaacga cgaagaaacc gtggcgctga ttgcgggtgg tcatacgctg ggtaaaaccc 4621 acggtgccgg tccgacatca aatgtaggtc ctgatccaga agctgcaccg attgaagaac 4681 aaggtttagg ttgggcgagc acttacggca gcggcgttgg cgcagatgcc attacctctg 4741 gtctggaagt agtctggacc cagacgccga cccagtggag caactatttc ttcgagaacc 4801 tgttcaagta tgagtgggta cagacccgca gcccggctgg cgcaatccag ttcgaagcgg 4861 tagacgcacc ggaaattatc ccggatccgt ttgatccgtc gaagaaacgt aaaccgacaa 4921 tgctggtgac cgacctgacg ctgcgttttg atcctgagtt cgagaagatc tctcgtcgtt 4981 tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg tgcctggttc aaactgacgc 5041 acagggatat ggggccgaaa tctcgctaca tcgggccgga agtgccgaaa gaagatctga 5101 tctggcaaga tccgctgccg cagccgatct acaacccgac cgagcaggac attatcgatc 5161 tgaaattcgc gattgcggat tctggtctgt ctgttagtga gctggtatcg gtggcctggg 5221 catctgcttc taccttccgt ggtggcgaca aacgcggtgg tgccaacggt gcgcgtctgg 5281 cattaatgcc gcagcgcgac tgggatgtga acgccgcagc cgttcgtgct ctgcctgttc 5341 tggagaaaat ccagaaagag tctggtaaag cctcgctggc ggatatcata gtgctggctg 5401 gtgtggttgg tgttgagaaa gccgcaagcg ccgcaggttt gagcattcat gtaccgtttg 5461 cgccgggtcg cgttgatgcg cgtcaggatc agactgacat tgagatgttt gagctgctgg 5521 agccaattgc tgacggtttc cgtaactatc gcgctcgtct ggacgtttcc accaccgagt 5581 cactgctgat cgacaaagca cagcaactga cgctgaccgc gccggaaatg actgcgctgg 5641 tgggcggcat gcgtgtactg ggtgccaact tcgatggcag caaaaacggc gtcttcactg 5701 accgcgttgg cgtattgagc aatgacttct tcgtgaactt gctggatatg cgttacgagt 5761 ggaaagcgac cgacgaatcg aaagagctgt tcgaaggccg tgaccgtgaa accggcgaag 5821 tgaaatttac ggccagccgt gcggatctgg tgtttggttc taactccgtc ctgcgtgcgg 5881 tggcggaagt ttacgccagt agcgatgccc acgagaagtt tgttaaagac ttcgtggcgg 5941 catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct ggctgctaac gacgaaaact 6001 acgctctggc tgcttaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 6061 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 6121 actggctcac cttcgggtgg gcctttctgc gtttata //