LOCUS BBa_K137079 3442 bp DNA linear 22-Dec-2024 DEFINITION AHL inducible expression of KatG ACCESSION BBa_K137079 VERSION BBa_K137079.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1..1082 /label=BBa_S03975 misc_feature 1286..1286 /label=C->T CDS 1089..3305 /label=BBa_K137068 stem_loop 3325..3368 /label=stem_loop misc_feature 3314..3393 /label=BBa_B0010 terminator 3314..3393 /label=BBa_B0010 stem_loop 3409..3428 /label=T7 TE misc_feature 3435..3435 /label=stop polyA_site 3429..3442 /label=polya misc_feature 3402..3442 /label=BBa_B0012 terminator 3402..3442 /label=BBa_B0012 misc_feature 1089..3442 /label=BBa_S04059 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gagcacgtca gacgatatcc ataacaccac agccactggc aaatgcccgt 1141 tccatcaggg cggtcacgac cagagtgcgg gggcgggcac aaccactcgc gactggtggc 1201 caaatcaact tcgtgttgac ctgttaaacc aacattctaa tcgttctaac ccactgggtg 1261 aggactttga ctaccgcaaa gaatttagca aattagatta ctacggcctg aaaaaagatc 1321 tgaaagccct gttgacagaa tctcaaccgt ggtggccagc cgactggggc agttacgccg 1381 gtctgtttat tcgtatggcc tggcacggcg cggggactta ccgttcaatc gatggacgcg 1441 gtggcgcggg tcgtggtcag caacgttttg caccgctgaa ctcctggccg gataacgtaa 1501 gcctcgataa agcgcgtcgc ctgttgtggc caatcaaaca gaaatatggt cagaaaatct 1561 cctgggccga cctgtttatc ctcgcgggta acgtggcgct agaaaactcc ggcttccgta 1621 ccttcggttt tggtgccggt cgtgaagacg tctgggaacc ggatctggat gttaactggg 1681 gtgatgaaaa agcctggctg actcaccgtc atccggaagc gctggcgaaa gcaccgctgg 1741 gtgcaaccga gatgggtctg atttacgtta acccggaagg cccggatcac agcggcgaac 1801 cgctttctgc ggcagcagct atccgcgcga ccttcggcaa catgggcatg aacgacgaag 1861 aaaccgtggc gctgattgcg ggtggtcata cgctgggtaa aacccacggt gccggtccga 1921 catcaaatgt aggtcctgat ccagaagctg caccgattga agaacaaggt ttaggttggg 1981 cgagcactta cggcagcggc gttggcgcag atgccattac ctctggtctg gaagtagtct 2041 ggacccagac gccgacccag tggagcaact atttcttcga gaacctgttc aagtatgagt 2101 gggtacagac ccgcagcccg gctggcgcaa tccagttcga agcggtagac gcaccggaaa 2161 ttatcccgga tccgtttgat ccgtcgaaga aacgtaaacc gacaatgctg gtgaccgacc 2221 tgacgctgcg ttttgatcct gagttcgaga agatctctcg tcgtttcctc aacgatccgc 2281 aggcgttcaa cgaagccttt gcccgtgcct ggttcaaact gacgcacagg gatatggggc 2341 cgaaatctcg ctacatcggg ccggaagtgc cgaaagaaga tctgatctgg caagatccgc 2401 tgccgcagcc gatctacaac ccgaccgagc aggacattat cgatctgaaa ttcgcgattg 2461 cggattctgg tctgtctgtt agtgagctgg tatcggtggc ctgggcatct gcttctacct 2521 tccgtggtgg cgacaaacgc ggtggtgcca acggtgcgcg tctggcatta atgccgcagc 2581 gcgactggga tgtgaacgcc gcagccgttc gtgctctgcc tgttctggag aaaatccaga 2641 aagagtctgg taaagcctcg ctggcggata tcatagtgct ggctggtgtg gttggtgttg 2701 agaaagccgc aagcgccgca ggtttgagca ttcatgtacc gtttgcgccg ggtcgcgttg 2761 atgcgcgtca ggatcagact gacattgaga tgtttgagct gctggagcca attgctgacg 2821 gtttccgtaa ctatcgcgct cgtctggacg tttccaccac cgagtcactg ctgatcgaca 2881 aagcacagca actgacgctg accgcgccgg aaatgactgc gctggtgggc ggcatgcgtg 2941 tactgggtgc caacttcgat ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat 3001 tgagcaatga cttcttcgtg aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg 3061 aatcgaaaga gctgttcgaa ggccgtgacc gtgaaaccgg cgaagtgaaa tttacggcca 3121 gccgtgcgga tctggtgttt ggttctaact ccgtcctgcg tgcggtggcg gaagtttacg 3181 ccagtagcga tgcccacgag aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga 3241 tgaacctcga ccgtttcgac ctgctggctg ctaacgacga aaactacgct ctggctgctt 3301 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3361 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3421 ggtgggcctt tctgcgttta ta //