LOCUS       BBa_K137084              958 bp    DNA     linear       22-Nov-2024
DEFINITION  Strong promoter + strong RBS + GFP with (CT)20 repeat after start 
            codon + double terminator
ACCESSION   BBa_K137084
VERSION     BBa_K137084.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..821
                     /label=BBa_K137082
     stem_loop       841..884
                     /label=stem_loop
     misc_feature    830..909
                     /label=BBa_B0010
     terminator      830..909
                     /label=BBa_B0010
     stem_loop       925..944
                     /label=T7 TE
     misc_feature    951..951
                     /label=stop
     polyA_site      945..958
                     /label=polya
     misc_feature    918..958
                     /label=BBa_B0012
     terminator      918..958
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgctctct ctctctctct ctctctctct ctctctctct ctctcgtaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg
      301 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca
      421 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact
      541 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa
      601 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
      781 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//