LOCUS       BBa_K137098              919 bp    DNA     linear       22-Dec-2024
DEFINITION  GFP device with optimized promoter with (A) repeat 18bp
ACCESSION   BBa_K137098
VERSION     BBa_K137098.1  
FEATURES             Location/Qualifiers
     promoter        1..36
                     /label=BBa_K137091
     misc_feature    49..52
                     /label=conserved
     RBS             45..56
                     /label=BBa_B0034
     CDS             63..782
                     /label=GFP protein
     CDS             63..782
                     /label=BBa_E0040
     stem_loop       802..845
                     /label=stem_loop
     misc_feature    791..870
                     /label=BBa_B0010
     terminator      791..870
                     /label=BBa_B0010
     stem_loop       886..905
                     /label=T7 TE
     misc_feature    912..912
                     /label=stop
     polyA_site      906..919
                     /label=polya
     misc_feature    879..919
                     /label=BBa_B0012
     terminator      879..919
                     /label=BBa_B0012
ORIGIN
        1 ttgacaaaaa aaaaaaaaaa aaaatataat gctagctact agagaaagag gagaaatact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
      781 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      841 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      901 gggcctttct gcgtttata
//