LOCUS BBa_K1371015 5235 bp DNA linear 23-Dec-2024 DEFINITION pT7+birA+accA+pccB+2TM-pT7+sfp+2TM ACCESSION BBa_K1371015 VERSION BBa_K1371015.1 FEATURES Location/Qualifiers promoter 1..23 /label=BBa_I719005 misc_feature 36..39 /label=conserved RBS 32..43 /label=BBa_B0034 CDS 52..1059 /label=birA CDS 52..1059 /label=BBa_K1371012 misc_feature 1072..1075 /label=conserved RBS 1068..1079 /label=BBa_B0034 CDS 1088..2863 /label=cds CDS 1088..2863 /label=BBa_K1371014 misc_feature 2876..2879 /label=conserved RBS 2872..2883 /label=BBa_B0034 CDS 2890..4485 /label=cds CDS 2890..4485 /label=BBa_K1371013 stem_loop 4501..4520 /label=T7 TE misc_feature 4527..4527 /label=stop polyA_site 4521..4534 /label=polya misc_feature 4494..4534 /label=BBa_B0012 terminator 4494..4534 /label=BBa_B0012 stem_loop 4550..4569 /label=T7 TE misc_feature 4576..4576 /label=stop polyA_site 4570..4583 /label=polya misc_feature 4543..4583 /label=BBa_B0012 terminator 4543..4583 /label=BBa_B0012 promoter 4592..4614 /label=BBa_I719005 misc_feature 4627..4630 /label=conserved RBS 4623..4634 /label=BBa_B0034 CDS 4641..5137 /label=sfp CDS 4641..5137 /label=BBa_K1371011 stem_loop 5153..5172 /label=T7 TE misc_feature 5179..5179 /label=stop polyA_site 5173..5186 /label=polya misc_feature 5146..5186 /label=BBa_B0012 terminator 5146..5186 /label=BBa_B0012 stem_loop 5202..5221 /label=T7 TE misc_feature 5228..5228 /label=stop polyA_site 5222..5235 /label=polya misc_feature 5195..5235 /label=BBa_B0012 terminator 5195..5235 /label=BBa_B0012 ORIGIN 1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga ggtaatacga 61 ctcactatag ggaaagagga gaaatactag agatgaagga taacaccgtg ccactgaaat 121 tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt gaaacgctgg 181 gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg ggcgttgatg 241 tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta cttaatgctg 301 aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt attgactcca 361 cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgcc tgtgttgcag 421 aataccagca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct tttggcgcaa 481 acttatattt gtcgatgttc tggcgtctgg aacaaggccc ggcggcggcg attggtttaa 541 gtctggttat cggtatcgtg atggcggaag tattacgcaa gctgggagca gataaagttc 601 gtgtcaaatg gcctaatgac ctctatctgc atgatcgcaa gctggcaggc attcttgtgg 661 agctgactgg caaaactggc gatgcggcgc aaatagtcat tggagccggg atcaacatgg 721 caatgcgccg tgttgaagag agtgtcgtta atcaggggtg gatcacgctg catgaagcgg 781 ggatcaatct cgatcgtaat acgttggcgg ccatgctaat acgtgaatta cgtgctgcgt 841 tggaactctt cgaacaagaa ggattggcac cttatctgtc gcgctgggaa aagctggata 901 attttattaa tcgcccagtg aaacttatca ttggtgataa agaaatattt ggcatttcac 961 gcggaataga caaacagggg gctttattac ttgagcagga tggaataata aaaccctgga 1021 tgggcggtga aatatccctg cgtagtgcag aaaaataatt actagagaaa gaggagaaat 1081 actagaggtg cgtaaagtgc tgatcgccaa tcgtggcgaa attgcagttc gtgtggcacg 1141 tgcatgccgc gatgccggta ttgccagcgt tgccgtgtac gccgatcctg atcgcgatgc 1201 cttacacgtg cgtgccgccg atgaagcatt cgcactgggt ggcgataccc cggccaccag 1261 ctatctggac atcgccaagg ttctgaaagc cgcccgcgag agtggtgccg atgccattca 1321 tcctggttac ggcttcctga gcgagaacgc agacttcgca caggccgttc tggatgcagg 1381 cctgatctgg attggtccgc cgccgcatgc cattcgtgat cgcggtgaaa aagtggccgc 1441 ccgtcacatt gcacagcgtg caggtgcccc gttagtggcc ggtacaccgg atccggtgag 1501 tggcgcagat gaggttgttg cattcgccaa ggaacacggt ctgcctattg ccatcaaagc 1561 cgcctttggc ggcggcggtc gtggtctgaa agttgcccgt accctggagg aagtgccgga 1621 actgtatgac agtgccgtgc gcgaagcagt ggcagcattt ggccgtggtg agtgctttgt 1681 tgaacgctac ctggataaac cgcgtcacgt ggagacccag tgcctggcag acacccatgg 1741 caatgtggtt gtggttagca cacgcgattg tagcctacag cgccgtcatc agaagctggt 1801 tgaagaggcg cctgccccgt ttctgagcga ggcccagaca gagcagctgt acagcagcag 1861 caaggccatc ctgaaagaag ccggctacgt tggtgccggc acagtggagt ttctggtggg 1921 catggatggc accatcagct ttctggaagt gaatacccgc ctacaggttg aacatccggt 1981 gacagaagag gtggcaggta ttgacctggt gcgtgagatg tttcgcattg ccgatggcga 2041 agagctgggt tacgatgacc cggccctgcg tggccatagc ttcgagttcc gtatcaacgg 2101 tgaccatccg ggccgtggtt tcctgcctgc accgggtacc gtgaccctgt ttgatgcccc 2161 taccggcccg ggcgtgcgtt tagatgcagg cgttgagagc ggtagtgtga ttggtccggc 2221 atgggatagc ctgctggcca agctgatcgt taccggtcgt acacgcgccg aagccttaca 2281 gcgtgccgca cgcgccttag acgagttcac agttgaaggc atggccaccg ccatcccgtt 2341 tcaccgtaca gtggtgcgtg atccggcatt tgccccggaa ctgaccggta gtaccgatcc 2401 gtttaccgtt cacacccgct ggatcgagac cgaatttgtg aatgagatta aaccttttac 2461 caccccggca gacacagaaa ccgatgaaga gagtggccgt gagacagttg tggtggaagt 2521 tggtggcaag cgcctggaag ttagcctgcc gagcagtctg ggtatgagtc tggcacgcac 2581 cggtttagca gcaggcgcac gcccgaaacg tcgtgccgca aagaagagtg gtccggcagc 2641 aagtggcgac accctggcca gcccgatgca gggcacaatt gtgaagatcg ccgtggaaga 2701 aggtcaggaa gttcaagagg gggatctgat tgttgtgctg gaagccatga agatggagca 2761 gccgctgaat gcccatcgta gcggcaccat caaaggtctg acagccgagg ttggtgccag 2821 cctgaccagc ggtgcagcca tttgtgagat taaagattaa taatactaga gaaagaggag 2881 aaatactaga tgagtgaacc ggaggagcag cagccggata tccacaccac agccggtaaa 2941 ctggcagatc tgcgccgtcg cattgaagag gccacccatg ccggtagcgc acgtgcagtg 3001 gaaaaacagc acgccaaagg caaactgaca gcccgcgaac gcattgacct gctgctggat 3061 gaaggtagct tcgtggagct ggatgagttt gcacgtcacc gtagcaccaa tttcggtctg 3121 gacgcaaatc gtccgtatgg cgatggcgtt gttaccggct atggtaccgt ggacggccgt 3181 ccggtggcag tgttcagcca ggatttcacc gtgtttggtg gtgccctggg tgaagtgtac 3241 ggccaaaaaa ttgtgaaggt tatggatttt gccttaaaaa ccggctgccc ggttgtgggc 3301 attaatgaca gcggtggtgc acgtattcaa gagggggttg ccagcctggg cgcctacggt 3361 gagatctttc gtcgtaacac ccatgccagt ggcgtgatcc ctcagattag cctggttgtg 3421 ggtccgtgtg ccggtggtgc cgtttatagc ccggcaatca ccgatttcac cgttatggtg 3481 gaccagacaa gccacatgtt tattaccggc cctgacgtga ttaagacagt tacaggcgag 3541 gatgtgggct ttgaggaact gggcggtgcc cgcacacaca atagcaccag cggtgttgcc 3601 catcatatgg ccggcgatga gaaggatgcc gtggagtacg ttaagcaact gctgagctac 3661 ttaccgagca ataacctgag cgaaccgccg gcctttccgg aagaggccga cctggcagtg 3721 accgatgagg atgccgaact ggataccatc gtgccggaca gcgccaacca gccgtacgac 3781 atgcatagcg tgatcgaaca cgtgctggat gatgcagagt tcttcgagac ccagccgctg 3841 tttgccccga atattctgac cggctttggt cgcgttgaag gccgtccggt gggcattgtg 3901 gcaaatcagc cgatgcagtt cgccggttgc ttagacatca ccgcaagcga gaaggccgcc 3961 cgttttgttc gtacctgcga cgcctttaat gtgccggtgc tgacctttgt tgacgttccg 4021 ggttttttac cgggcgtgga tcaggagcac gacggcatca ttcgtcgcgg tgccaaactg 4081 atcttcgcct atgcagaagc caccgttccg ctgatcaccg tgatcacacg caaagccttt 4141 ggtggcgcct atgacgttat gggcagcaaa catctgggcg ccgatctgaa tctggcctgg 4201 ccgaccgcac agattgcagt gatgggcgca cagggcgcag tgaatattct gcaccgccgt 4261 accattgcag atgccggtga tgacgccgaa gccacccgtg cccgtctgat ccaggagtat 4321 gaggacgcct tactgaaccc gtataccgca gcagagcgcg gttacgtgga tgcagtgatc 4381 atgccgagcg atacccgtcg ccatatcgtg cgtggtctgc gtcaactgcg caccaaacgt 4441 gaaagcctgc ctccgaagaa gcacggtaat attcctctgt aataatacta gagtcacact 4501 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcacactg gctcaccttc 4561 gggtgggcct ttctgcgttt atatactaga gtaatacgac tcactatagg gagatactag 4621 agaaagagga gaaatactag atgaagattt acggaattta tatggaccgc ccgctttcac 4681 aggaagaaaa tgaacggttc atgtctttca tatcacctga aaaacgggag aaatgccgga 4741 gattttatca taaagaagat gctcaccgca ccctgctggg agatgtgctc gttcgctcag 4801 tcataagcag gcagtatcag tggacaaatc cgatatccgc tttagcacgc aggaatacgg 4861 gaagccgtgc atccctgatc ttcccgacgc tcatttcaac atttctcact ccggacgctg 4921 ggtcatttgc gcgtttgatt cacagccgat cggcatagat atcgaaaaaa cgaaaccgat 4981 cagccttgag atcgccaagc gcttcttttc aaaaacagag tacagcgacc ttttagcaaa 5041 agacaaggac gagcagacag actattttta tcatctatgg tcaatgaaag aaagctttat 5101 caaacaagga aggcaaaggc ttatcgcttc cgcttgatac tagagtcaca ctggctcacc 5161 ttcgggtggg cctttctgcg tttatatact agagtcacac tggctcacct tcgggtgggc 5221 ctttctgcgt ttata //