BBa_K1371012 1 BBa_K1371012 birA 2014-10-07T11:00:00Z 2015-05-08T01:10:10Z aa aa false false _1748_ 0 16007 9 It's complicated false aa false Qifeng Tan annotation2404167 1 birA range2404167 1 1 1008 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K1371014 1 BBa_K1371014 accA2 2014-10-06T11:00:00Z 2015-05-08T01:10:10Z aa aa false false _1748_ 0 16007 9 It's complicated false aa false Qifeng Tan annotation2398020 1 cds range2398020 1 1 1776 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1371013 1 BBa_K1371013 pccB 2014-10-04T11:00:00Z 2015-05-08T01:10:10Z aa aa false false _1748_ 0 16007 9 It's complicated false aa false Qifeng Tan annotation2395464 1 cds range2395464 1 1 1596 BBa_K1371015 1 BBa_K1371015 pT7+birA+accA+pccB+2TM-pT7+sfp+2TM 2014-10-15T11:00:00Z 2015-05-08T01:10:10Z aa aa false false _1748_ 0 16007 9 Not in stock false aa false Qifeng Tan component2421076 1 BBa_B0012 component2421067 1 BBa_K1371012 component2421073 1 BBa_B0034 component2421069 1 BBa_B0034 component2421080 1 BBa_B0012 component2421084 1 BBa_I719005 component2421063 1 BBa_I719005 component2421092 1 BBa_B0012 component2421086 1 BBa_B0034 component2421071 1 BBa_K1371014 component2421065 1 BBa_B0034 component2421088 1 BBa_B0012 component2421087 1 BBa_K1371011 component2421075 1 BBa_K1371013 annotation2421076 1 BBa_B0012 range2421076 1 4494 4534 annotation2421092 1 BBa_B0012 range2421092 1 5195 5235 annotation2421086 1 BBa_B0034 range2421086 1 4623 4634 annotation2421069 1 BBa_B0034 range2421069 1 1068 1079 annotation2421080 1 BBa_B0012 range2421080 1 4543 4583 annotation2421065 1 BBa_B0034 range2421065 1 32 43 annotation2421088 1 BBa_B0012 range2421088 1 5146 5186 annotation2421087 1 BBa_K1371011 range2421087 1 4641 5137 annotation2421067 1 BBa_K1371012 range2421067 1 52 1059 annotation2421063 1 BBa_I719005 range2421063 1 1 23 annotation2421073 1 BBa_B0034 range2421073 1 2872 2883 annotation2421075 1 BBa_K1371013 range2421075 1 2890 4485 annotation2421071 1 BBa_K1371014 range2421071 1 1088 2863 annotation2421084 1 BBa_I719005 range2421084 1 4592 4614 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1371011 1 BBa_K1371011 sfp 2014-10-15T11:00:00Z 2015-05-08T01:10:10Z aa aa false false _1748_ 0 16007 9 Not in stock false aa false Yuxin Chen annotation2428829 1 sfp range2428829 1 1 497 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K1371011_sequence 1 atgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagtggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaaggaaggcaaaggcttatcgcttccgcttga BBa_K1371015_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagaggtaatacgactcactatagggaaagaggagaaatactagagatgaaggataacaccgtgccactgaaattgattgccctgttagcgaacggtgaatttcactctggcgagcagttgggtgaaacgctgggaatgagccgggcggctattaataaacacattcagacactgcgtgactggggcgttgatgtctttaccgttccgggtaaaggatacagcctgcctgagcccatccagttacttaatgctgaacagatattgggtcagctggatggcggtagtgtagccgtgctgccagttattgactccacgaatcagtaccttcttgatcgtatcggagagcttaaatcgggcgatgcctgtgttgcagaataccagcaggctggccgtggtcgccgggggcggaaatggttttcgccttttggcgcaaacttatatttgtcgatgttctggcgtctggaacaaggcccggcggcggcgattggtttaagtctggttatcggtatcgtgatggcggaagtattacgcaagctgggagcagataaagttcgtgtcaaatggcctaatgacctctatctgcatgatcgcaagctggcaggcattcttgtggagctgactggcaaaactggcgatgcggcgcaaatagtcattggagccgggatcaacatggcaatgcgccgtgttgaagagagtgtcgttaatcaggggtggatcacgctgcatgaagcggggatcaatctcgatcgtaatacgttggcggccatgctaatacgtgaattacgtgctgcgttggaactcttcgaacaagaaggattggcaccttatctgtcgcgctgggaaaagctggataattttattaatcgcccagtgaaacttatcattggtgataaagaaatatttggcatttcacgcggaatagacaaacagggggctttattacttgagcaggatggaataataaaaccctggatgggcggtgaaatatccctgcgtagtgcagaaaaataattactagagaaagaggagaaatactagaggtgcgtaaagtgctgatcgccaatcgtggcgaaattgcagttcgtgtggcacgtgcatgccgcgatgccggtattgccagcgttgccgtgtacgccgatcctgatcgcgatgccttacacgtgcgtgccgccgatgaagcattcgcactgggtggcgataccccggccaccagctatctggacatcgccaaggttctgaaagccgcccgcgagagtggtgccgatgccattcatcctggttacggcttcctgagcgagaacgcagacttcgcacaggccgttctggatgcaggcctgatctggattggtccgccgccgcatgccattcgtgatcgcggtgaaaaagtggccgcccgtcacattgcacagcgtgcaggtgccccgttagtggccggtacaccggatccggtgagtggcgcagatgaggttgttgcattcgccaaggaacacggtctgcctattgccatcaaagccgcctttggcggcggcggtcgtggtctgaaagttgcccgtaccctggaggaagtgccggaactgtatgacagtgccgtgcgcgaagcagtggcagcatttggccgtggtgagtgctttgttgaacgctacctggataaaccgcgtcacgtggagacccagtgcctggcagacacccatggcaatgtggttgtggttagcacacgcgattgtagcctacagcgccgtcatcagaagctggttgaagaggcgcctgccccgtttctgagcgaggcccagacagagcagctgtacagcagcagcaaggccatcctgaaagaagccggctacgttggtgccggcacagtggagtttctggtgggcatggatggcaccatcagctttctggaagtgaatacccgcctacaggttgaacatccggtgacagaagaggtggcaggtattgacctggtgcgtgagatgtttcgcattgccgatggcgaagagctgggttacgatgacccggccctgcgtggccatagcttcgagttccgtatcaacggtgaccatccgggccgtggtttcctgcctgcaccgggtaccgtgaccctgtttgatgcccctaccggcccgggcgtgcgtttagatgcaggcgttgagagcggtagtgtgattggtccggcatgggatagcctgctggccaagctgatcgttaccggtcgtacacgcgccgaagccttacagcgtgccgcacgcgccttagacgagttcacagttgaaggcatggccaccgccatcccgtttcaccgtacagtggtgcgtgatccggcatttgccccggaactgaccggtagtaccgatccgtttaccgttcacacccgctggatcgagaccgaatttgtgaatgagattaaaccttttaccaccccggcagacacagaaaccgatgaagagagtggccgtgagacagttgtggtggaagttggtggcaagcgcctggaagttagcctgccgagcagtctgggtatgagtctggcacgcaccggtttagcagcaggcgcacgcccgaaacgtcgtgccgcaaagaagagtggtccggcagcaagtggcgacaccctggccagcccgatgcagggcacaattgtgaagatcgccgtggaagaaggtcaggaagttcaagagggggatctgattgttgtgctggaagccatgaagatggagcagccgctgaatgcccatcgtagcggcaccatcaaaggtctgacagccgaggttggtgccagcctgaccagcggtgcagccatttgtgagattaaagattaataatactagagaaagaggagaaatactagatgagtgaaccggaggagcagcagccggatatccacaccacagccggtaaactggcagatctgcgccgtcgcattgaagaggccacccatgccggtagcgcacgtgcagtggaaaaacagcacgccaaaggcaaactgacagcccgcgaacgcattgacctgctgctggatgaaggtagcttcgtggagctggatgagtttgcacgtcaccgtagcaccaatttcggtctggacgcaaatcgtccgtatggcgatggcgttgttaccggctatggtaccgtggacggccgtccggtggcagtgttcagccaggatttcaccgtgtttggtggtgccctgggtgaagtgtacggccaaaaaattgtgaaggttatggattttgccttaaaaaccggctgcccggttgtgggcattaatgacagcggtggtgcacgtattcaagagggggttgccagcctgggcgcctacggtgagatctttcgtcgtaacacccatgccagtggcgtgatccctcagattagcctggttgtgggtccgtgtgccggtggtgccgtttatagcccggcaatcaccgatttcaccgttatggtggaccagacaagccacatgtttattaccggccctgacgtgattaagacagttacaggcgaggatgtgggctttgaggaactgggcggtgcccgcacacacaatagcaccagcggtgttgcccatcatatggccggcgatgagaaggatgccgtggagtacgttaagcaactgctgagctacttaccgagcaataacctgagcgaaccgccggcctttccggaagaggccgacctggcagtgaccgatgaggatgccgaactggataccatcgtgccggacagcgccaaccagccgtacgacatgcatagcgtgatcgaacacgtgctggatgatgcagagttcttcgagacccagccgctgtttgccccgaatattctgaccggctttggtcgcgttgaaggccgtccggtgggcattgtggcaaatcagccgatgcagttcgccggttgcttagacatcaccgcaagcgagaaggccgcccgttttgttcgtacctgcgacgcctttaatgtgccggtgctgacctttgttgacgttccgggttttttaccgggcgtggatcaggagcacgacggcatcattcgtcgcggtgccaaactgatcttcgcctatgcagaagccaccgttccgctgatcaccgtgatcacacgcaaagcctttggtggcgcctatgacgttatgggcagcaaacatctgggcgccgatctgaatctggcctggccgaccgcacagattgcagtgatgggcgcacagggcgcagtgaatattctgcaccgccgtaccattgcagatgccggtgatgacgccgaagccacccgtgcccgtctgatccaggagtatgaggacgccttactgaacccgtataccgcagcagagcgcggttacgtggatgcagtgatcatgccgagcgatacccgtcgccatatcgtgcgtggtctgcgtcaactgcgcaccaaacgtgaaagcctgcctccgaagaagcacggtaatattcctctgtaataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgtctttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagtggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggacgctgggtcatttgcgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaaggaaggcaaaggcttatcgcttccgcttgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1371013_sequence 1 atgagtgaaccggaggagcagcagccggatatccacaccacagccggtaaactggcagatctgcgccgtcgcattgaagaggccacccatgccggtagcgcacgtgcagtggaaaaacagcacgccaaaggcaaactgacagcccgcgaacgcattgacctgctgctggatgaaggtagcttcgtggagctggatgagtttgcacgtcaccgtagcaccaatttcggtctggacgcaaatcgtccgtatggcgatggcgttgttaccggctatggtaccgtggacggccgtccggtggcagtgttcagccaggatttcaccgtgtttggtggtgccctgggtgaagtgtacggccaaaaaattgtgaaggttatggattttgccttaaaaaccggctgcccggttgtgggcattaatgacagcggtggtgcacgtattcaagagggggttgccagcctgggcgcctacggtgagatctttcgtcgtaacacccatgccagtggcgtgatccctcagattagcctggttgtgggtccgtgtgccggtggtgccgtttatagcccggcaatcaccgatttcaccgttatggtggaccagacaagccacatgtttattaccggccctgacgtgattaagacagttacaggcgaggatgtgggctttgaggaactgggcggtgcccgcacacacaatagcaccagcggtgttgcccatcatatggccggcgatgagaaggatgccgtggagtacgttaagcaactgctgagctacttaccgagcaataacctgagcgaaccgccggcctttccggaagaggccgacctggcagtgaccgatgaggatgccgaactggataccatcgtgccggacagcgccaaccagccgtacgacatgcatagcgtgatcgaacacgtgctggatgatgcagagttcttcgagacccagccgctgtttgccccgaatattctgaccggctttggtcgcgttgaaggccgtccggtgggcattgtggcaaatcagccgatgcagttcgccggttgcttagacatcaccgcaagcgagaaggccgcccgttttgttcgtacctgcgacgcctttaatgtgccggtgctgacctttgttgacgttccgggttttttaccgggcgtggatcaggagcacgacggcatcattcgtcgcggtgccaaactgatcttcgcctatgcagaagccaccgttccgctgatcaccgtgatcacacgcaaagcctttggtggcgcctatgacgttatgggcagcaaacatctgggcgccgatctgaatctggcctggccgaccgcacagattgcagtgatgggcgcacagggcgcagtgaatattctgcaccgccgtaccattgcagatgccggtgatgacgccgaagccacccgtgcccgtctgatccaggagtatgaggacgccttactgaacccgtataccgcagcagagcgcggttacgtggatgcagtgatcatgccgagcgatacccgtcgccatatcgtgcgtggtctgcgtcaactgcgcaccaaacgtgaaagcctgcctccgaagaagcacggtaatattcctctgtaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1371012_sequence 1 gtaatacgactcactatagggaaagaggagaaatactagagatgaaggataacaccgtgccactgaaattgattgccctgttagcgaacggtgaatttcactctggcgagcagttgggtgaaacgctgggaatgagccgggcggctattaataaacacattcagacactgcgtgactggggcgttgatgtctttaccgttccgggtaaaggatacagcctgcctgagcccatccagttacttaatgctgaacagatattgggtcagctggatggcggtagtgtagccgtgctgccagttattgactccacgaatcagtaccttcttgatcgtatcggagagcttaaatcgggcgatgcctgtgttgcagaataccagcaggctggccgtggtcgccgggggcggaaatggttttcgccttttggcgcaaacttatatttgtcgatgttctggcgtctggaacaaggcccggcggcggcgattggtttaagtctggttatcggtatcgtgatggcggaagtattacgcaagctgggagcagataaagttcgtgtcaaatggcctaatgacctctatctgcatgatcgcaagctggcaggcattcttgtggagctgactggcaaaactggcgatgcggcgcaaatagtcattggagccgggatcaacatggcaatgcgccgtgttgaagagagtgtcgttaatcaggggtggatcacgctgcatgaagcggggatcaatctcgatcgtaatacgttggcggccatgctaatacgtgaattacgtgctgcgttggaactcttcgaacaagaaggattggcaccttatctgtcgcgctgggaaaagctggataattttattaatcgcccagtgaaacttatcattggtgataaagaaatatttggcatttcacgcggaatagacaaacagggggctttattacttgagcaggatggaataataaaaccctggatgggcggtgaaatatccctgcgtagtgcagaaaaataat BBa_K1371014_sequence 1 gtgcgtaaagtgctgatcgccaatcgtggcgaaattgcagttcgtgtggcacgtgcatgccgcgatgccggtattgccagcgttgccgtgtacgccgatcctgatcgcgatgccttacacgtgcgtgccgccgatgaagcattcgcactgggtggcgataccccggccaccagctatctggacatcgccaaggttctgaaagccgcccgcgagagtggtgccgatgccattcatcctggttacggcttcctgagcgagaacgcagacttcgcacaggccgttctggatgcaggcctgatctggattggtccgccgccgcatgccattcgtgatcgcggtgaaaaagtggccgcccgtcacattgcacagcgtgcaggtgccccgttagtggccggtacaccggatccggtgagtggcgcagatgaggttgttgcattcgccaaggaacacggtctgcctattgccatcaaagccgcctttggcggcggcggtcgtggtctgaaagttgcccgtaccctggaggaagtgccggaactgtatgacagtgccgtgcgcgaagcagtggcagcatttggccgtggtgagtgctttgttgaacgctacctggataaaccgcgtcacgtggagacccagtgcctggcagacacccatggcaatgtggttgtggttagcacacgcgattgtagcctacagcgccgtcatcagaagctggttgaagaggcgcctgccccgtttctgagcgaggcccagacagagcagctgtacagcagcagcaaggccatcctgaaagaagccggctacgttggtgccggcacagtggagtttctggtgggcatggatggcaccatcagctttctggaagtgaatacccgcctacaggttgaacatccggtgacagaagaggtggcaggtattgacctggtgcgtgagatgtttcgcattgccgatggcgaagagctgggttacgatgacccggccctgcgtggccatagcttcgagttccgtatcaacggtgaccatccgggccgtggtttcctgcctgcaccgggtaccgtgaccctgtttgatgcccctaccggcccgggcgtgcgtttagatgcaggcgttgagagcggtagtgtgattggtccggcatgggatagcctgctggccaagctgatcgttaccggtcgtacacgcgccgaagccttacagcgtgccgcacgcgccttagacgagttcacagttgaaggcatggccaccgccatcccgtttcaccgtacagtggtgcgtgatccggcatttgccccggaactgaccggtagtaccgatccgtttaccgttcacacccgctggatcgagaccgaatttgtgaatgagattaaaccttttaccaccccggcagacacagaaaccgatgaagagagtggccgtgagacagttgtggtggaagttggtggcaagcgcctggaagttagcctgccgagcagtctgggtatgagtctggcacgcaccggtttagcagcaggcgcacgcccgaaacgtcgtgccgcaaagaagagtggtccggcagcaagtggcgacaccctggccagcccgatgcagggcacaattgtgaagatcgccgtggaagaaggtcaggaagttcaagagggggatctgattgttgtgctggaagccatgaagatggagcagccgctgaatgcccatcgtagcggcaccatcaaaggtctgacagccgaggttggtgccagcctgaccagcggtgcagccatttgtgagattaaagattaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z