LOCUS       BBa_K137102             1569 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong promoter + strong RBS + tetA with (AGTC)9 repeat after 
            start codon + double terminator
ACCESSION   BBa_K137102
VERSION     BBa_K137102.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1432
                     /label=BBa_K137100
     stem_loop       1452..1495
                     /label=stem_loop
     misc_feature    1441..1520
                     /label=BBa_B0010
     terminator      1441..1520
                     /label=BBa_B0010
     stem_loop       1536..1555
                     /label=T7 TE
     misc_feature    1562..1562
                     /label=stop
     polyA_site      1556..1569
                     /label=polya
     misc_feature    1529..1569
                     /label=BBa_B0012
     terminator      1529..1569
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagtcag tcagtcagtc agtcagtcag tcagtcagtc agtcaaatct aacaatgcgc
      121 tcatcgtcat tctcggcacc gtcaccctgg acgctgtagg cataggcttg gttatgccgg
      181 tactgccggg cctcttgcgg gatatcgtcc attccgacag tattgccagt cactatggcg
      241 tgctgcttgc gctctatgcg ttgatgcaat ttctttgcgc acccgttctc ggagccctgt
      301 ccgaccgctt tggccgccgt ccagtcctgc tcgcttcgct ccttggagcc actatcgact
      361 acgcgatcat ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggcgg
      421 gcatcacggg tgccacaggt gcggttgctg gtgcctatat cgccgacatc accgatgggg
      481 aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
      541 gccccgtggc cgggggactg ttgggtgcca tctccttgca tgcaccattc cttgcggcgg
      601 cggtgctcaa cggcctcaac ctcctcctgg gctgcttcct tatgcaggaa tcgcataagg
      661 gagagcgccg tccgatgccc ttgcgtgcct tcaatccagt cagctccttc cggtgggcgc
      721 ggggcatgac tatcgtcgcc gcacttatga ctgttttctt tatcatgcaa ctcgtaggac
      781 aggttccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
      841 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
      901 cgggccccgc caccaaacgt ttcggcgaga agcaggccat tatcgcgggc atggcggccg
      961 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgcgg ctggatggcc ttccccatta
     1021 tgattcttct cgcttccggc ggcatcggta tgcccgcgtt gcaggccatg ctgtcccgcc
     1081 aagtagatga cgaccatcag ggacagcttc aagggtcgct cgcggctctt accagcctca
     1141 cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga
     1201 acgggttggc atggattgta ggtgccgccc tttaccttgt ctgcctcccc gcgttgcgtc
     1261 gcggtgcatg gagccgggcc acctcgacct aataatacta gagccaggca tcaaataaaa
     1321 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1381 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1441 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1501 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1561 gcgtttata
//