LOCUS       BBa_K137107             3309 bp    DNA     linear       08-Jun-2024
DEFINITION  Strong promoter + strong RBS + LacZ with (AGTC)10 repeat after 
            start codon + double terminator
ACCESSION   BBa_K137107
VERSION     BBa_K137107.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..3172
                     /label=BBa_K137105
     stem_loop       3192..3235
                     /label=stem_loop
     misc_feature    3181..3260
                     /label=BBa_B0010
     terminator      3181..3260
                     /label=BBa_B0010
     stem_loop       3276..3295
                     /label=T7 TE
     misc_feature    3302..3302
                     /label=stop
     polyA_site      3296..3309
                     /label=polya
     misc_feature    3269..3309
                     /label=BBa_B0012
     terminator      3269..3309
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagtcag tcagtcagtc agtcagtcag tcagtcagtc accatgatta cggattcact
      121 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      181 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      241 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
      301 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
      361 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
      421 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
      481 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa
      541 ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt
      601 gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat
      661 ggtgctgcgc tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg
      721 cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt
      781 tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg
      841 cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt
      901 cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga
      961 tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa
     1021 tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc
     1081 ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa
     1141 gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat
     1201 ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt
     1261 gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct
     1321 gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac
     1381 cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga
     1441 tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa
     1501 tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga
     1561 aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga
     1621 tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc
     1681 tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg
     1741 cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt
     1801 ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc
     1861 ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt
     1921 ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt
     1981 ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag
     2041 cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga
     2101 agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca
     2161 gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc
     2221 atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag
     2281 tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt
     2341 ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca
     2401 gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc
     2461 accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt
     2521 cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc
     2581 agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa
     2641 aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac
     2701 cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca
     2761 gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc
     2821 cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac
     2881 cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg
     2941 cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact
     3001 gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga
     3061 cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt
     3121 tcagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat aatactagag
     3181 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3241 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     3301 gcgtttata
//