LOCUS       BBa_K137108             3313 bp    DNA     linear       22-Dec-2024
DEFINITION  Strong promoter + strong RBS + LacZ with (AGTC)9 repeat after 
            start codon + double terminator
ACCESSION   BBa_K137108
VERSION     BBa_K137108.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..3176
                     /label=BBa_K137106
     stem_loop       3196..3239
                     /label=stem_loop
     misc_feature    3185..3264
                     /label=BBa_B0010
     terminator      3185..3264
                     /label=BBa_B0010
     stem_loop       3280..3299
                     /label=T7 TE
     misc_feature    3306..3306
                     /label=stop
     polyA_site      3300..3313
                     /label=polya
     misc_feature    3273..3313
                     /label=BBa_B0012
     terminator      3273..3313
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgagtcag tcagtcagtc agtcagtcag tcagtcagtc agtcaccatg attacggatt
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
      301 cagaagcggt gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg
      361 tcccctcaaa ctggcagatg cacggttacg atgcgcccat ctacaccaac gtgacctatc
      421 ccattacggt caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca
      481 catttaatgt tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg
      541 ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc
      601 gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg
      661 tgatggtgct gcgctggagt gacggcagtt atctggaaga tcaggatatg tggcggatga
      721 gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc
      781 atgttgccac tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga
      841 tgtgcggcga gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc
      901 aggtcgccag cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg
      961 ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc
     1021 cgaatctcta tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag
     1081 aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg
     1141 gcaagccgtt gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg
     1201 tcatggatga gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg
     1261 ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg
     1321 gcctgtatgt ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc
     1381 tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc
     1441 gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg
     1501 ctaatcacga cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt
     1561 atgaaggcgg cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg
     1621 tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc
     1681 tacctggaga gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc
     1741 ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct
     1801 tcgtctggga ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt
     1861 cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc
     1921 tggtctttgc cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt
     1981 ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc
     2041 atagcgataa cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg
     2101 gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac
     2161 cgcagccgga gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga
     2221 ccgcatggtc agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc
     2281 tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg
     2341 atttttgcat cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt
     2401 cacagatgtg gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc
     2461 gtgcaccgct ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct
     2521 gggtcgaacg ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca
     2581 cggcagatac acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg
     2641 ggaaaacctt atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga
     2701 ttaccgttga tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact
     2761 gccagctggc gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact
     2821 atcccgaccg ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt
     2881 ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt
     2941 atggcccaca ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc
     3001 aactgatgga aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata
     3061 tcgacggttt ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg
     3121 aatttcagct gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataatact
     3181 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     3241 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     3301 ttctgcgttt ata
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