BBa_K137109 1 BBa_K137109 pabBj + b0015 2008-08-21T11:00:00Z 2015-05-08T01:10:11Z The original source of the gene is from the genomic DNA of Lactococcus lactis. This is the complete construct of pabB from the para-aminobenzoic acid synthesis pathway with the strong constitutive promoter j23100, the strong RBS b0034, and the double terminator b0015. false false _187_ 0 2987 9 Not in stock false We wanted a functional plasmid with just pabB to test for para-aminobenzoic acid levels during overexpression. false Victoria Hsiao component1973961 1 BBa_J23100 component1973967 1 BBa_B0012 component1973964 1 BBa_K137006 component1973965 1 BBa_B0010 component1973963 1 BBa_B0034 annotation1973963 1 BBa_B0034 range1973963 1 44 55 annotation1973965 1 BBa_B0010 range1973965 1 1960 2039 annotation1973961 1 BBa_J23100 range1973961 1 1 35 annotation1973964 1 BBa_K137006 range1973964 1 62 1951 annotation1973967 1 BBa_B0012 range1973967 1 2048 2088 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K137006 1 BBa_K137006 pabB (from pABA synthesis) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome PabB from L.lactis is a fusion gene of both the usual pabB and pabC in the pABA synthesis pathway from chorismate. false false _187_ 0 2987 9 It's complicated false The start codon of pabB in L.lactis is TTG, which cannot be used in E.coli. So we designed the primer such that the first T was omitted and the A from the xba1 primer make an ATG start codon. false Victoria Hsiao annotation1971718 1 Changed T to A range1971718 1 1 1 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K137006_sequence 1 atgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K137109_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z