BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K137005 1 pabA pabA (from pABA synthesis) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome pabA is a gene involved in para-aminobenzoic acid (pABA) synthesis from chorismate. PABA is necessary for the synthesis of folate. false false _187_ 0 2987 9 It's complicated false We decided to clone the pABA synthesis genes separately (even though they reside side by side) because L.lactis uses start codons (ttg) that E.coli cannot, and so we need to change the start codon for pabB to atg. false Victoria Hsiao BBa_K137110 1 BBa_K137110 pabA + pabB complete pABA construct 2008-08-21T11:00:00Z 2015-05-08T01:10:11Z Both genes were extracted from the genomic DNA of lactococcus lactis. This construct contains both genes from the para-aminobenzoic acid synthesis pathway, pabA and pabB. The construct contains the constitutive strong promoter j23100, strong RBS b0034, pabA, b0034, pabB, and then the double terminator b0015. false false _187_ 0 2987 9 Not in stock false We wanted to test constitutive overexpression of the para-aminobenzoic acid synthesis genes to see if that would improve total folate overexpression levels in E. coli. false Victoria Hsiao component1973977 1 BBa_K137006 component1973973 1 BBa_B0034 component1973974 1 BBa_K137005 component1973980 1 BBa_B0012 component1973971 1 BBa_J23100 component1973976 1 BBa_B0034 component1973978 1 BBa_B0010 annotation1973974 1 BBa_K137005 range1973974 1 62 646 annotation1973978 1 BBa_B0010 range1973978 1 2571 2650 annotation1973980 1 BBa_B0012 range1973980 1 2659 2699 annotation1973973 1 BBa_B0034 range1973973 1 44 55 annotation1973971 1 BBa_J23100 range1973971 1 1 35 annotation1973977 1 BBa_K137006 range1973977 1 673 2562 annotation1973976 1 BBa_B0034 range1973976 1 655 666 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K137006 1 BBa_K137006 pabB (from pABA synthesis) 2008-06-19T11:00:00Z 2015-05-08T01:10:08Z L.lactis genome PabB from L.lactis is a fusion gene of both the usual pabB and pabC in the pABA synthesis pathway from chorismate. false false _187_ 0 2987 9 It's complicated false The start codon of pabB in L.lactis is TTG, which cannot be used in E.coli. So we designed the primer such that the first T was omitted and the A from the xba1 primer make an ATG start codon. false Victoria Hsiao annotation1971718 1 Changed T to A range1971718 1 1 1 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K137006_sequence 1 atgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K137005_sequence 1 atgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K137110_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaattgctattaattgataattatgattcttttacttatttgcttgcacagtattttgaggaattagattgtagtgtaacagtggttaatgaccaagataaaatgagtcagaaaattagaatttctccggattttatttgtgaaaattatgatgcgattatcatttctccaggtccaaaaacaccaaaagaagccgtttttagtagggatgtagttcaactatacgctggaaaaattcctatgttaggaatttgcttgggtcagcaagtcattgctgaatgttttggaggaaatgttgttcttggtgagaggccaatgcatggaaaaatttctgtcattcgtcataattatcaaggaatttttaaaggacttccacaaaatttgaaagttgcacgttatcattcgttgattgtcgataaactacctgatgattttgaaattgatgcgcagagtgaggatggtgttattcaggccattcatcagccaaaattaaaattgtgggctttacaatttcacccagaaagtttagttactgaatatggtcatgaaatgttaaataattttttgaaagtggtgtaataatactagagaaagaggagaaatactagatgaaggaatttataataaaaaatacggatatttggaaaattttcctaaaatattatcggtcagatgaagaaattgtttttttacatagtagtcaggtgaccgaaaatgagcattatagtattttagctcataaaccttataaaaaggtttcaaaatataagggtcaagtgttttttaatggtgaaaaaaagaagttcaattttttagatgcggtagatttgctgaaggatgagagagttgaacgacctaaaaattggcctttttatccagaattattgggttttgtaagttatgagcaagatccggcttgttttgcagcttatgatgaagttttactttttgaccatagaacaaaactgttacgtgtcgtgcaatttgaacaaactgatggccaatattggctgactgaatcggaagaaattgaagtggattcagagattgagttcgatgttcagaatggcataggggctgtttttattgaccaaacgaggcaagaatatattgcttcaataaaaaaaatacaagattatatgaaggctggcgatatttatgttgccaatttgacgcaacaatttgaaatttggtccgatcaaaaacctattgatgtttttaagaaaacaaggaaacaaattcctgctcctttctcatcatttttacaatatcctgaatggaaaatgacgcaaatttcaagttctgttgaacgctttgtttctattcataatggagcattaatttcaaaacccattaaaggaacaattgccagaggagaaaatgttgtagctgatagattacaaaagaaaatactttccgacagtagtaaagaacggtctgagttactgatggtgacggatttattgcgtaatgatattgcccgaattagtcaacctttttctttgtctgtccctaaatttgcagaaattgaaactttttctcatgttcatcaactggtgacttcaataaaaagtagaattaaagaagatttgactttttcagaatttatgacggctttatttccgggaggttcaatcacaggtacacctaagaagcgggcaatggagattattaaggaagttgaaaaacaacctagaggaatttatacagggatgcagggttggttaagtcgagaaatggatttggatatgaacatcgtgattcggacattagttcatgacggtgagcattatcagcttggtgtaggcggtggaattacgttcgagagcgaagcagaagcagagttttcagagattttactaaaagcaaaaccctttttagatatattaggcctgaaagatgttccaagtattttatttacaacaggccttgttaaaaatggtgaattgttaaatttagagggtcatgtcaatcgcttgaaaaaacaataccatcatgcagatttggaagaaaaattgagaatatttgcccaaaaggttactgacggggttttacgtatcagtactgacggagattctttgacccctgggattcgtcagttgacccattcaaatgaggcctatcgagttaaactgtcgtcaattaatgacaaacctagtcttttgtctaactttaaactttcaggcccagattttcaaaaggtctttcgtcaggaagtcttagaagcgaaaaaagaagggtttcaagatattctatttcatacagatggtttgatcagtgaattatcaatcggaaattttgtggcaaaaaaggggaatcagtacgaaacgccagccaaatatgctctaaaaggaacttttttagatttatttgcgaaaaatcatacgctaatatataaagatattgcgatttctgatttgaaaacttacgatcgtttttatatgacaaatgctgtcagaggattagttgaaataaaaattgatggaatttcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z