LOCUS BBa_K137117 2830 bp DNA linear 22-Dec-2024 DEFINITION AHL induced expression of rcsA with constitutive cI ACCESSION BBa_K137117 VERSION BBa_K137117.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 CDS 1089..1712 /label=BBa_K137113 stem_loop 1732..1775 /label=stem_loop misc_feature 1721..1800 /label=BBa_B0010 terminator 1721..1800 /label=BBa_B0010 stem_loop 1816..1835 /label=T7 TE misc_feature 1842..1842 /label=stop polyA_site 1836..1849 /label=polya misc_feature 1809..1849 /label=BBa_B0012 terminator 1809..1849 /label=BBa_B0012 misc_feature 1071..1849 /label=BBa_S04080 misc_feature 1..1849 /label=BBa_K137114 promoter 1858..1892 /label=BBa_J23113 misc_feature 1905..1908 /label=conserved RBS 1901..1912 /label=BBa_B0034 CDS 1922..2629 /label=cI lambda misc_feature 2630..2662 /label=LVA misc_feature 2669..2693 /label=Help:Barcodes CDS 1919..2668 /label=BBa_C0051 stem_loop 2713..2756 /label=stem_loop misc_feature 2702..2781 /label=BBa_B0010 terminator 2702..2781 /label=BBa_B0010 stem_loop 2797..2816 /label=T7 TE misc_feature 2823..2823 /label=stop polyA_site 2817..2830 /label=polya misc_feature 2790..2830 /label=BBa_B0012 terminator 2790..2830 /label=BBa_B0012 misc_feature 1901..2830 /label=BBa_P0451 misc_feature 1858..2830 /label=BBa_K137115 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg 1141 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg 1201 accttgccat agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt 1261 tcatccacga tgcttctaac agtcagcgta tcaagctcat cattaatcaa catcccaata 1321 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca 1381 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg 1441 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat 1501 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa 1561 tctctgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac 1621 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg 1681 tgactaatgg tatttttgtc aacatgcgct aatactagag ccaggcatca aataaaacga 1741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagctg 1861 atggctagct cagtcctagg gattatgcta gctactagag aaagaggaga aatactagat 1921 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1981 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 2041 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 2101 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 2161 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2221 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 2281 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2341 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2401 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2461 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2521 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2581 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2641 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2701 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2761 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2821 tgcgtttata //