LOCUS       BBa_K137119             1557 bp    DNA     linear       22-Dec-2024
DEFINITION  TetR Cassette
ACCESSION   BBa_K137119
VERSION     BBa_K137119.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     promoter        138..226
                     /label=tetR promoter
     CDS             227..1420
                     /label=tetA
     misc_feature    138..1420
                     /label=BBa_P1005
     stem_loop       1440..1483
                     /label=stem_loop
     misc_feature    1429..1508
                     /label=BBa_B0010
     terminator      1429..1508
                     /label=BBa_B0010
     stem_loop       1524..1543
                     /label=T7 TE
     misc_feature    1550..1550
                     /label=stop
     polyA_site      1544..1557
                     /label=polya
     misc_feature    1517..1557
                     /label=BBa_B0012
     terminator      1517..1557
                     /label=BBa_B0012
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagaggag attctcatgt ttgacagctt atcatcgata agctttaatg
      181 cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa
      241 tgcgctcatc gtcattctcg gcaccgtcac cctggacgct gtaggcatag gcttggttat
      301 gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagtattg ccagtcacta
      361 tggcgtgctg cttgcgctct atgcgttgat gcaatttctt tgcgcacccg ttctcggagc
      421 cctgtccgac cgctttggcc gccgtccagt cctgctcgct tcgctccttg gagccactat
      481 cgactacgcg atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt
      541 ggcgggcatc acgggtgcca caggtgcggt tgctggtgcc tatatcgccg acatcaccga
      601 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
      661 ggcaggcccc gtggccgggg gactgttggg tgccatctcc ttgcatgcac cattccttgc
      721 ggcggcggtg ctcaacggcc tcaacctcct cctgggctgc ttccttatgc aggaatcgca
      781 taagggagag cgccgtccga tgcccttgcg tgccttcaat ccagtcagct ccttccggtg
      841 ggcgcggggc atgactatcg tcgccgcact tatgactgtt ttctttatca tgcaactcgt
      901 aggacaggtt ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
      961 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     1021 cgtcacgggc cccgccacca aacgtttcgg cgagaagcag gccattatcg cgggcatggc
     1081 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgcggctgga tggccttccc
     1141 cattatgatt cttctcgctt ccggcggcat cggtatgccc gcgttgcagg ccatgctgtc
     1201 ccgccaagta gatgacgacc atcagggaca gcttcaaggg tcgctcgcgg ctcttaccag
     1261 cctcacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac
     1321 atggaacggg ttggcatgga ttgtaggtgc cgccctttac cttgtctgcc tccccgcgtt
     1381 gcgtcgcggt gcatggagcc gggccacctc gacctaataa tactagagcc aggcatcaaa
     1441 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1501 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//