LOCUS       BBa_K137120             2966 bp    DNA     linear       15-May-2024
DEFINITION  LamB and TetA expression plasmid
ACCESSION   BBa_K137120
VERSION     BBa_K137120.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..1401
                     /label=BBa_K137112
     stem_loop       1421..1464
                     /label=stem_loop
     misc_feature    1410..1489
                     /label=BBa_B0010
     terminator      1410..1489
                     /label=BBa_B0010
     stem_loop       1505..1524
                     /label=T7 TE
     misc_feature    1531..1531
                     /label=stop
     polyA_site      1525..1538
                     /label=polya
     misc_feature    1498..1538
                     /label=BBa_B0012
     terminator      1498..1538
                     /label=BBa_B0012
     promoter        1547..1635
                     /label=tetR promoter
     CDS             1636..2829
                     /label=tetA
     misc_feature    1547..2829
                     /label=BBa_P1005
     stem_loop       2849..2892
                     /label=stem_loop
     misc_feature    2838..2917
                     /label=BBa_B0010
     terminator      2838..2917
                     /label=BBa_B0010
     stem_loop       2933..2952
                     /label=T7 TE
     misc_feature    2959..2959
                     /label=stop
     polyA_site      2953..2966
                     /label=polya
     misc_feature    2926..2966
                     /label=BBa_B0012
     terminator      2926..2966
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagtcacaca ggaaagtact
       61 agatgatgat tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg ggcgtaatgt
      121 ctgctcaggc aatggctgtt gatttccacg gctatgcacg ttccggtatt ggttggacag
      181 gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa taccgtcttg
      241 gcaacgaatg tgaaacttat gctgaattaa aattgggtca ggaagtgtgg aaagagggcg
      301 ataagagctt ctatttcgac actaacgtgg cctattccgt cgcacaacag aatgactggg
      361 aagctaccga tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg atcgaatggc
      421 tgccaggctc caccatctgg gcaggtaagc gcttctacca acgtcatgac gttcatatga
      481 tcgacttcta ctactgggat atttctggtc ctggtgccgg tctggaaaac atcgatgttg
      541 gcttcggtaa actctctctg gcagcaaccc gctcctctga agctggtggt tcttcctctt
      601 tcgccagcaa caatatttat gactatacca acgaaaccgc gaacgacgtt ttcgatgtgc
      661 gtttagcgca gatggaaatc aacccgggcg gcacattaga actgggtgtc gactacggtc
      721 gtgccaactt gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac ggctggttat
      781 tcactgctga acatactcag agtgtcctga agggctttaa caagtttgtt gttcagtacg
      841 ctactgactg acctcgcagg gtaaagggct gtcgcagggt tctggcgttg catttgataa
      901 cgaaaaattt gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg
      961 tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa
     1021 ctgggataac gacaacggca ccaagtggtg gaccgtcggt attcgcccga tgtacaagtg
     1081 gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac
     1141 cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag
     1201 catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg
     1261 gggttacgac tacaccggta acgctgataa caacgcgaac ttcggcaaag ccgttcctgc
     1321 tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca
     1381 gatggaaatc tggtggtaat atactagagc caggcatcaa ataaaacgaa aggctcagtc
     1441 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1501 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggaga ttctcatgtt
     1561 tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa ttgctaacgc
     1621 agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcattctcgg caccgtcacc
     1681 ctggacgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     1741 gtccattccg acagtattgc cagtcactat ggcgtgctgc ttgcgctcta tgcgttgatg
     1801 caatttcttt gcgcacccgt tctcggagcc ctgtccgacc gctttggccg ccgtccagtc
     1861 ctgctcgctt cgctccttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     1921 ctgtggattc tctacgccgg acgcatcgtg gcgggcatca cgggtgccac aggtgcggtt
     1981 gctggtgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     2041 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggt
     2101 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctcctc
     2161 ctgggctgct tccttatgca ggaatcgcat aagggagagc gccgtccgat gcccttgcgt
     2221 gccttcaatc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     2281 atgactgttt tctttatcat gcaactcgta ggacaggttc cggcagcgct ctgggtcatt
     2341 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     2401 ggaatcttgc acgccctcgc tcaagccttc gtcacgggcc ccgccaccaa acgtttcggc
     2461 gagaagcagg ccattatcgc gggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     2521 ttcgcgacgc gcggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     2581 ggtatgcccg cgttgcaggc catgctgtcc cgccaagtag atgacgacca tcagggacag
     2641 cttcaagggt cgctcgcggc tcttaccagc ctcacttcga tcattggacc gctgatcgtc
     2701 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggtgcc
     2761 gccctttacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     2821 acctaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2881 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2941 ttcgggtggg cctttctgcg tttata
//