LOCUS       BBa_K137121             2964 bp    DNA     linear       22-Dec-2024
DEFINITION  lamB and tetR cassette Medium Promoter+Weak RBS
ACCESSION   BBa_K137121
VERSION     BBa_K137121.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     CDS             61..1399
                     /label=BBa_K137112
     stem_loop       1419..1462
                     /label=stem_loop
     misc_feature    1408..1487
                     /label=BBa_B0010
     terminator      1408..1487
                     /label=BBa_B0010
     stem_loop       1503..1522
                     /label=T7 TE
     misc_feature    1529..1529
                     /label=stop
     polyA_site      1523..1536
                     /label=polya
     misc_feature    1496..1536
                     /label=BBa_B0012
     terminator      1496..1536
                     /label=BBa_B0012
     promoter        1545..1633
                     /label=tetR promoter
     CDS             1634..2827
                     /label=tetA
     misc_feature    1545..2827
                     /label=BBa_P1005
     stem_loop       2847..2890
                     /label=stem_loop
     misc_feature    2836..2915
                     /label=BBa_B0010
     terminator      2836..2915
                     /label=BBa_B0010
     stem_loop       2931..2950
                     /label=T7 TE
     misc_feature    2957..2957
                     /label=stop
     polyA_site      2951..2964
                     /label=polya
     misc_feature    2924..2964
                     /label=BBa_B0012
     terminator      2924..2964
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagtcacaca ggactactag
       61 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
      121 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ttggacaggt
      181 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
      241 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
      301 aagagcttct atttcgacac taacgtggcc tattccgtcg cacaacagaa tgactgggaa
      361 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
      421 ccaggctcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
      481 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
      541 ttcggtaaac tctctctggc agcaacccgc tcctctgaag ctggtggttc ttcctctttc
      601 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
      661 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
      721 gccaacttgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
      781 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
      841 actgactgac ctcgcagggt aaagggctgt cgcagggttc tggcgttgca tttgataacg
      901 aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg
      961 cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag gatatcaact
     1021 gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg tacaagtgga
     1081 cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg
     1141 gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct ggcgacagca
     1201 tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg
     1261 gttacgacta caccggtaac gctgataaca acgcgaactt cggcaaagcc gttcctgctg
     1321 atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc ggtgcccaga
     1381 tggaaatctg gtggtaatat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1441 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1501 ctggctcacc ttcgggtggg cctttctgcg tttatatact agaggagatt ctcatgtttg
     1561 acagcttatc atcgataagc tttaatgcgg tagtttatca cagttaaatt gctaacgcag
     1621 tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc attctcggca ccgtcaccct
     1681 ggacgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc gggatatcgt
     1741 ccattccgac agtattgcca gtcactatgg cgtgctgctt gcgctctatg cgttgatgca
     1801 atttctttgc gcacccgttc tcggagccct gtccgaccgc tttggccgcc gtccagtcct
     1861 gctcgcttcg ctccttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct
     1921 gtggattctc tacgccggac gcatcgtggc gggcatcacg ggtgccacag gtgcggttgc
     1981 tggtgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     2041 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggtgc
     2101 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctcctcct
     2161 gggctgcttc cttatgcagg aatcgcataa gggagagcgc cgtccgatgc ccttgcgtgc
     2221 cttcaatcca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     2281 gactgttttc tttatcatgc aactcgtagg acaggttccg gcagcgctct gggtcatttt
     2341 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     2401 aatcttgcac gccctcgctc aagccttcgt cacgggcccc gccaccaaac gtttcggcga
     2461 gaagcaggcc attatcgcgg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     2521 cgcgacgcgc ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     2581 tatgcccgcg ttgcaggcca tgctgtcccg ccaagtagat gacgaccatc agggacagct
     2641 tcaagggtcg ctcgcggctc ttaccagcct cacttcgatc attggaccgc tgatcgtcac
     2701 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggtgccgc
     2761 cctttacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     2821 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2941 cgggtgggcc tttctgcgtt tata
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