LOCUS       BBa_K137122             2829 bp    DNA     linear       10-Dec-2025
DEFINITION  lamB and TetR cassette Weak Promoter+Medium RBS
ACCESSION   BBa_K137122
VERSION     BBa_K137122.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..1401
                     /label=BBa_K137112
     promoter        1410..1498
                     /label=tetR promoter
     CDS             1499..2692
                     /label=tetA
     misc_feature    1410..2692
                     /label=BBa_P1005
     stem_loop       2712..2755
                     /label=stem_loop
     misc_feature    2701..2780
                     /label=BBa_B0010
     terminator      2701..2780
                     /label=BBa_B0010
     stem_loop       2796..2815
                     /label=T7 TE
     misc_feature    2822..2822
                     /label=stop
     polyA_site      2816..2829
                     /label=polya
     misc_feature    2789..2829
                     /label=BBa_B0012
     terminator      2789..2829
                     /label=BBa_B0012
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgatgat tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg ggcgtaatgt
      121 ctgctcaggc aatggctgtt gatttccacg gctatgcacg ttccggtatt ggttggacag
      181 gtagcggcgg tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa taccgtcttg
      241 gcaacgaatg tgaaacttat gctgaattaa aattgggtca ggaagtgtgg aaagagggcg
      301 ataagagctt ctatttcgac actaacgtgg cctattccgt cgcacaacag aatgactggg
      361 aagctaccga tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg atcgaatggc
      421 tgccaggctc caccatctgg gcaggtaagc gcttctacca acgtcatgac gttcatatga
      481 tcgacttcta ctactgggat atttctggtc ctggtgccgg tctggaaaac atcgatgttg
      541 gcttcggtaa actctctctg gcagcaaccc gctcctctga agctggtggt tcttcctctt
      601 tcgccagcaa caatatttat gactatacca acgaaaccgc gaacgacgtt ttcgatgtgc
      661 gtttagcgca gatggaaatc aacccgggcg gcacattaga actgggtgtc gactacggtc
      721 gtgccaactt gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac ggctggttat
      781 tcactgctga acatactcag agtgtcctga agggctttaa caagtttgtt gttcagtacg
      841 ctactgactg acctcgcagg gtaaagggct gtcgcagggt tctggcgttg catttgataa
      901 cgaaaaattt gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg
      961 tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa
     1021 ctgggataac gacaacggca ccaagtggtg gaccgtcggt attcgcccga tgtacaagtg
     1081 gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac
     1141 cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag
     1201 catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg
     1261 gggttacgac tacaccggta acgctgataa caacgcgaac ttcggcaaag ccgttcctgc
     1321 tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca
     1381 gatggaaatc tggtggtaat atactagagg agattctcat gtttgacagc ttatcatcga
     1441 taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat
     1501 gaaatctaac aatgcgctca tcgtcattct cggcaccgtc accctggacg ctgtaggcat
     1561 aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagtat
     1621 tgccagtcac tatggcgtgc tgcttgcgct ctatgcgttg atgcaatttc tttgcgcacc
     1681 cgttctcgga gccctgtccg accgctttgg ccgccgtcca gtcctgctcg cttcgctcct
     1741 tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga ttctctacgc
     1801 cggacgcatc gtggcgggca tcacgggtgc cacaggtgcg gttgctggtg cctatatcgc
     1861 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     1921 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggtgccatct ccttgcatgc
     1981 accattcctt gcggcggcgg tgctcaacgg cctcaacctc ctcctgggct gcttccttat
     2041 gcaggaatcg cataagggag agcgccgtcc gatgcccttg cgtgccttca atccagtcag
     2101 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg ttttctttat
     2161 catgcaactc gtaggacagg ttccggcagc gctctgggtc attttcggcg aggaccgctt
     2221 tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
     2281 cgctcaagcc ttcgtcacgg gccccgccac caaacgtttc ggcgagaagc aggccattat
     2341 cgcgggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgcggctg
     2401 gatggccttc cccattatga ttcttctcgc ttccggcggc atcggtatgc ccgcgttgca
     2461 ggccatgctg tcccgccaag tagatgacga ccatcaggga cagcttcaag ggtcgctcgc
     2521 ggctcttacc agcctcactt cgatcattgg accgctgatc gtcacggcga tttatgccgc
     2581 ctcggcgagc acatggaacg ggttggcatg gattgtaggt gccgcccttt accttgtctg
     2641 cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctaat aatactagag
     2701 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2761 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2821 gcgtttata
//