LOCUS       BBa_K137123             2827 bp    DNA     linear       22-Dec-2024
DEFINITION  lamB and tetR cassette Weak Promoter+Weak RBS
ACCESSION   BBa_K137123
VERSION     BBa_K137123.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23113
     RBS             44..54
                     /label=RBS-4\Weaker
     RBS             50..53
                     /label=RBS
     misc_feature    44..54
                     /label=BBa_B0033
     RBS             44..54
                     /label=BBa_B0033
     CDS             61..1399
                     /label=BBa_K137112
     promoter        1408..1496
                     /label=tetR promoter
     CDS             1497..2690
                     /label=tetA
     misc_feature    1408..2690
                     /label=BBa_P1005
     stem_loop       2710..2753
                     /label=stem_loop
     misc_feature    2699..2778
                     /label=BBa_B0010
     terminator      2699..2778
                     /label=BBa_B0010
     stem_loop       2794..2813
                     /label=T7 TE
     misc_feature    2820..2820
                     /label=stop
     polyA_site      2814..2827
                     /label=polya
     misc_feature    2787..2827
                     /label=BBa_B0012
     terminator      2787..2827
                     /label=BBa_B0012
ORIGIN
        1 ctgatggcta gctcagtcct agggattatg ctagctacta gagtcacaca ggactactag
       61 atgatgatta ctctgcgcaa acttcctctg gcggttgccg tcgcagcggg cgtaatgtct
      121 gctcaggcaa tggctgttga tttccacggc tatgcacgtt ccggtattgg ttggacaggt
      181 agcggcggtg aacaacagtg tttccagact accggtgctc aaagtaaata ccgtcttggc
      241 aacgaatgtg aaacttatgc tgaattaaaa ttgggtcagg aagtgtggaa agagggcgat
      301 aagagcttct atttcgacac taacgtggcc tattccgtcg cacaacagaa tgactgggaa
      361 gctaccgatc cggccttccg tgaagcaaac gtgcagggta aaaacctgat cgaatggctg
      421 ccaggctcca ccatctgggc aggtaagcgc ttctaccaac gtcatgacgt tcatatgatc
      481 gacttctact actgggatat ttctggtcct ggtgccggtc tggaaaacat cgatgttggc
      541 ttcggtaaac tctctctggc agcaacccgc tcctctgaag ctggtggttc ttcctctttc
      601 gccagcaaca atatttatga ctataccaac gaaaccgcga acgacgtttt cgatgtgcgt
      661 ttagcgcaga tggaaatcaa cccgggcggc acattagaac tgggtgtcga ctacggtcgt
      721 gccaacttgc gtgataacta tcgtctggtt gatggcgcat cgaaagacgg ctggttattc
      781 actgctgaac atactcagag tgtcctgaag ggctttaaca agtttgttgt tcagtacgct
      841 actgactgac ctcgcagggt aaagggctgt cgcagggttc tggcgttgca tttgataacg
      901 aaaaatttgc ctacaatatc aacaacaacg gtcacatgct gcgtatcctc gaccacggtg
      961 cgatctccat gggcgacaac tgggacatga tgtacgtggg tatgtaccag gatatcaact
     1021 gggataacga caacggcacc aagtggtgga ccgtcggtat tcgcccgatg tacaagtgga
     1081 cgccaatcat gagcaccgtg atggaaatcg gctacgacaa cgtcgaatcc cagcgcaccg
     1141 gcgacaagaa caatcagtac aaaattaccc tcgcacaaca atggcaggct ggcgacagca
     1201 tctggtcacg cccggctatt cgtgtcttcg caacctacgc caagtgggat gagaaatggg
     1261 gttacgacta caccggtaac gctgataaca acgcgaactt cggcaaagcc gttcctgctg
     1321 atttcaacgg cggcagcttc ggtcgtggcg acagcgacga gtggaccttc ggtgcccaga
     1381 tggaaatctg gtggtaatat actagaggag attctcatgt ttgacagctt atcatcgata
     1441 agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
     1501 aatctaacaa tgcgctcatc gtcattctcg gcaccgtcac cctggacgct gtaggcatag
     1561 gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagtattg
     1621 ccagtcacta tggcgtgctg cttgcgctct atgcgttgat gcaatttctt tgcgcacccg
     1681 ttctcggagc cctgtccgac cgctttggcc gccgtccagt cctgctcgct tcgctccttg
     1741 gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatt ctctacgccg
     1801 gacgcatcgt ggcgggcatc acgggtgcca caggtgcggt tgctggtgcc tatatcgccg
     1861 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     1921 tgggtatggt ggcaggcccc gtggccgggg gactgttggg tgccatctcc ttgcatgcac
     1981 cattccttgc ggcggcggtg ctcaacggcc tcaacctcct cctgggctgc ttccttatgc
     2041 aggaatcgca taagggagag cgccgtccga tgcccttgcg tgccttcaat ccagtcagct
     2101 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtt ttctttatca
     2161 tgcaactcgt aggacaggtt ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     2221 gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     2281 ctcaagcctt cgtcacgggc cccgccacca aacgtttcgg cgagaagcag gccattatcg
     2341 cgggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgcggctgga
     2401 tggccttccc cattatgatt cttctcgctt ccggcggcat cggtatgccc gcgttgcagg
     2461 ccatgctgtc ccgccaagta gatgacgacc atcagggaca gcttcaaggg tcgctcgcgg
     2521 ctcttaccag cctcacttcg atcattggac cgctgatcgt cacggcgatt tatgccgcct
     2581 cggcgagcac atggaacggg ttggcatgga ttgtaggtgc cgccctttac cttgtctgcc
     2641 tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctaataa tactagagcc
     2701 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2761 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2821 gtttata
//