LOCUS       BBa_K137129             1848 bp    DNA     linear       23-Dec-2024
DEFINITION  AHL inducible RcsA (weak RBS)
ACCESSION   BBa_K137129
VERSION     BBa_K137129.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    1008..1027
                     /label=LuxR/HSL
     promoter        1027..1032
                     /label=-35
     promoter        1049..1054
                     /label=-10
     misc_feature    1060..1060
                     /label=start
     misc_feature    1008..1062
                     /label=BBa_R0062
     promoter        1008..1062
                     /label=BBa_R0062
     RBS             1071..1081
                     /label=RBS-4\Weaker
     RBS             1077..1080
                     /label=RBS
     misc_feature    1071..1081
                     /label=BBa_B0033
     RBS             1071..1081
                     /label=BBa_B0033
     CDS             1088..1711
                     /label=BBa_K137113
     stem_loop       1731..1774
                     /label=stem_loop
     misc_feature    1720..1799
                     /label=BBa_B0010
     terminator      1720..1799
                     /label=BBa_B0010
     stem_loop       1815..1834
                     /label=T7 TE
     misc_feature    1841..1841
                     /label=stop
     polyA_site      1835..1848
                     /label=polya
     misc_feature    1808..1848
                     /label=BBa_B0012
     terminator      1808..1848
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg
     1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag tcacacagga
     1081 ctactagatg tcaacgatta ttatggattt atgtagttac acccgactag gtttaaccgg
     1141 gtatctgttg agtagagggg ttaaaaaaag agaaatcaac gacattgaaa ccgttgatga
     1201 ccttgccata gcttgtgatt cacagcgccc ttcagtggtg tttattaatg aggactgttt
     1261 catccacgat gcttctaaca gtcagcgtat caagctcatc attaatcaac atcccaatac
     1321 gttatttatc gtttttatgg caattgccaa tgttcatttt gatgaatatc tattggtcag
     1381 aaaaaattta ttgatcagtt ctaaatcgat taaaccggaa tctctcgacg atatccttgg
     1441 cgatattctg aaaaaagaga caacgataac ctcgttttta aatatgccga cgttatcatt
     1501 gagccgaacc gaatcgagta tgttgcgaat gtggatggca ggtcagggaa ccattcaaat
     1561 ctctgaccaa atgaatatca aagccaagac cgtttcatcg cataaaggta atattaaacg
     1621 taagatcaaa acgcataata aacaggttat ctaccatgtc gtccgactga cggataatgt
     1681 gactaatggt atttttgtca acatgcgcta atactagagc caggcatcaa ataaaacgaa
     1741 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1801 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//