LOCUS       BBa_K137132             2345 bp    DNA     linear       14-May-2024
DEFINITION  Lactose inducible GFP expression
ACCESSION   BBa_K137132
VERSION     BBa_K137132.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     promoter        1360..1462
                     /label=BBa_K137125
     misc_feature    44..1462
                     /label=BBa_S04105
     misc_feature    1..1462
                     /label=BBa_S04106
     misc_feature    1475..1478
                     /label=conserved
     RBS             1471..1482
                     /label=BBa_B0034
     CDS             1489..2208
                     /label=GFP protein
     CDS             1489..2208
                     /label=BBa_E0040
     stem_loop       2228..2271
                     /label=stem_loop
     misc_feature    2217..2296
                     /label=BBa_B0010
     terminator      2217..2296
                     /label=BBa_B0010
     stem_loop       2312..2331
                     /label=T7 TE
     misc_feature    2338..2338
                     /label=stop
     polyA_site      2332..2345
                     /label=polya
     misc_feature    2305..2345
                     /label=BBa_B0012
     terminator      2305..2345
                     /label=BBa_B0012
     misc_feature    1471..2345
                     /label=BBa_I13504
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagt acaacgtcgt gttagttgca
     1381 attgtgagcg gataacaatt gacttgtgag cggataacaa tgatacttcg tgcaattttt
     1441 aaaattaaag gcgttaccca actactagag aaagaggaga aatactagat gcgtaaagga
     1501 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1561 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1621 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1681 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1741 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1801 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1861 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1921 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1981 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2041 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2101 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2161 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     2221 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2281 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2341 ttata
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