LOCUS       BBa_K1373004            1942 bp    DNA     linear       30-Dec-2025
DEFINITION  nadE and oprF with strong promoter and strong RBS
ACCESSION   BBa_K1373004
VERSION     BBa_K1373004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             56..883
                     /label=nadE
     CDS             56..883
                     /label=BBa_K1373000
     misc_feature    1..883
                     /label=BBa_K1373003
     RBS             884..889
                     /label=strong RBS
     RBS             884..889
                     /label=BBa_K103015
     CDS             890..1942
                     /label=oprF
     CDS             890..1942
                     /label=BBa_K1373005
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaaatgac
       61 attgcaacaa caaataataa aggcgctggg cgcaaaaccg cagattaatg ctgaagagga
      121 aattcgtcgt agtgtcgatt ttctgaaaag ctacctgcaa acttatccgt tcattaaatc
      181 actggtgctc gggatcagcg gcggtcagga ctccacgctt gccggaaagc tgtgccagat
      241 ggcgattaat gagctgcgcc tggaaaccgg caacgaatca ctgcaattta ttgccgtacg
      301 cctgccctat ggtgttcagg ccgacgaaca agattgccag gatgccattg cctttattca
      361 accggatcgc gtattaaccg ttaatatcaa gggcgcggta ttggccagcg aacaggcatt
      421 gcgggaagca ggcattgaac tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg
      481 gatgaaagca caatatagca ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca
      541 tgcagcagaa gccattaccg gattcttcac taaatatggt gacggcggta cggacattaa
      601 tccgctgtat cgtctcaaca aacgtcaggg taaacagtta ctggcggcat tagcttgccc
      661 ggaacacctt tataagaaag cgccaacggc cgatctggaa gatgatcgcc cttctctgcc
      721 agatgaagtg gcactcggcg tgacctatga caatatcgac gactatctgg aagggaaaaa
      781 cgtacctcaa caggtcgcca gaacaataga gaactggtat ctgaaaaccg aacataaacg
      841 ccgtccgcca attaccgttt tcgatgattt ctggaaaaag taaaggagga tgaaactgaa
      901 gaacacctta ggcgttgtca tcggctcgct ggttgccgct tcggcaatga acgcctttgc
      961 ccagggccag aactcggtag agatcgaagc cttcggcaag cgctacttca ccgacagcgt
     1021 tcgcaacatg aagaacgcgg acctgtacgg cggctcgatc ggttacttcc tgaccgacga
     1081 cgtcgagctg gcgctgtcct acggtgagta ccatgacgtt cgtggcacct acgaaaccgg
     1141 caacaagaag gtccacggca acctgacctc cctggacgcc atctaccact tcggtacccc
     1201 gggcgtaggt ctgcgtccgt acgtgtcggc tggtctggct caccagaaca tcaccaacat
     1261 caacagcgac agccaaggcc gtcagcagat gaccatggcc aacatcggcg ctggtctgaa
     1321 gtactacttc accgagaact tcttcgccaa ggccagcctc gacggccagt acggtctgga
     1381 gaagcgtgac aacggtcacc agggcgagtg gatggctggc ctgggcgtcg gcttcaactt
     1441 cggtggttcg aaagccgctc cggctccgga accggttgcc gacgtttgct ccgactccga
     1501 caacgacggc gtttgcgaca acgtcgacaa gtgcccggat accccggcca acgtcaccgt
     1561 tgacgccaac ggctgcccgg ctgtcgccga agtcgtacgc gtacagctgg acgtgaagtt
     1621 cgacttcgac aagtccaagg tcaaagagaa cagctacgct gacatcaaga acctggctga
     1681 cttcatgaag cagtacccgt ccacttccac caccgttgaa ggtcacaccg actccgtcgg
     1741 caccgacgct tacaaccaga agctgtccga gcgtcgtgcc aacgccgttc gtgacgtact
     1801 ggtcaacgag tacggtgtag aaggtggtcg cgtgaacgct gttggttacg gcgagtcccg
     1861 cccggttgcc gacaacgcca ccgctgaagg ccgcgctatc aaccgtcgcg ttgaagccga
     1921 agtagaagct gaagccaagt aa
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