##gff-version 3
##sequence-region BBa_K1375001 1 925
BBa_K1375001	.	engineered_region	1	788	.	+	0	ID=BBa_K575014;Name=BBa_K575014
BBa_K1375001	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071;Parent=BBa_K575014
BBa_K1375001	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1375001	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1375001	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1375001	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1375001	.	ribosome_entry_site	62	76	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K575014
BBa_K1375001	.	ribosome_entry_site	69	73	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1375001	.	engineered_region	62	76	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1375001	.	ribosome_entry_site	62	76	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1375001	.	CDS	83	788	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K575014
BBa_K1375001	.	engineered_tag	764	788	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1375001	.	CDS	83	757	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1375001	.	terminator	797	925	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1375001	.	terminator	797	876	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375001	.	engineered_region	797	876	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375001	.	stem_loop	808	851	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375001	.	terminator	885	925	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375001	.	stop_codon	918	918	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375001	.	polyA_site	912	925	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375001	.	stem_loop	892	911	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375001	.	engineered_region	885	925	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1375001
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatac
tagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata