##gff-version 3
##sequence-region BBa_K1375008 1 946
BBa_K1375008	.	engineered_region	63	946	.	+	0	ID=BBa_I1466;Name=BBa_I1466
BBa_K1375008	.	terminator	906	946	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I1466
BBa_K1375008	.	polyA_site	933	946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375008	.	stem_loop	913	932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375008	.	engineered_region	906	946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375008	.	stop_codon	939	939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375008	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I1466
BBa_K1375008	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375008	.	CDS	81	809	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_I1466
BBa_K1375008	.	terminator	818	897	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I1466
BBa_K1375008	.	stem_loop	829	872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375008	.	engineered_region	818	897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375008	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1375008	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1375008	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1375008	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1375008	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1375008	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
>BBa_K1375008
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata