LOCUS       BBa_K1375011             948 bp    DNA     linear       01-Jul-2024
DEFINITION  Plac+RBS+LuxI+DT
ACCESSION   BBa_K1375011
VERSION     BBa_K1375011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..811
                     /label=LuxI
     CDS             227..811
                     /label=BBa_C0161
     misc_feature    1..811
                     /label=BBa_K084012
     stem_loop       831..874
                     /label=stem_loop
     misc_feature    820..899
                     /label=BBa_B0010
     terminator      820..899
                     /label=BBa_B0010
     stem_loop       915..934
                     /label=T7 TE
     misc_feature    941..941
                     /label=stop
     polyA_site      935..948
                     /label=polya
     misc_feature    908..948
                     /label=BBa_B0012
     terminator      908..948
                     /label=BBa_B0012
     terminator      820..948
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaattaata atactagagc caggcatcaa ataaaacgaa
      841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//