LOCUS       BBa_K1375017            1767 bp    DNA     linear       06-Nov-2024
DEFINITION  Pcin+RBS+CinR+RBS+LasI+DT
ACCESSION   BBa_K1375017
VERSION     BBa_K1375017.1  
FEATURES             Location/Qualifiers
     RBS             226..231
                     /label=embedded RBS
     stem_loop       35..74
                     /label=stem_loop
     stem_loop       91..136
                     /label=stem_loop
     promoter        1..220
                     /label=unidentified/uncharacterized CinR dependent
     promoter        1..231
                     /label=BBa_R0077
     CDS             238..960
                     /label=cinR
     misc_feature    961..993
                     /label=LVA
     misc_feature    1000..1024
                     /label=Help:Barcodes
     CDS             238..1024
                     /label=BBa_C0077
     RBS             1033..1047
                     /label=RBS-1\Strong
     RBS             1040..1044
                     /label=RBS
     misc_feature    1033..1047
                     /label=BBa_B0030
     RBS             1033..1047
                     /label=BBa_B0030
     CDS             1054..1656
                     /label=LasI
     misc_feature    1657..1689
                     /label=LVA
     misc_feature    1696..1720
                     /label=Help:Barcodes
     CDS             1054..1720
                     /label=BBa_C0078
     misc_feature    1729..1767
                     /label=B1006
     stem_loop       1738..1759
                     /label=modified thr terminator
     polyA_site      1760..1767
                     /label=PolyA
     polyA_site      1729..1737
                     /label=PolyA
     terminator      1729..1767
                     /label=BBa_B1006
     misc_feature    1033..1767
                     /label=BBa_K081016
ORIGIN
        1 ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg ggtcgcgatc tttcagattc
       61 gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt atcgcaaaac cgctgcacac
      121 ttttgcgcga catgctctga tccccctcat ctgggggggc ctatctgagg gaatttccga
      181 tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa aacgctggag gtactagatg
      241 attgagaata cctatagcga aaagttcgag tccgcgttcg aacagatcaa ggcggcggcc
      301 aacgtggatg ccgccatccg tattctccag gcggaatata acctcgattt cgtcacctac
      361 catctcgccc agacgatcgc gagcaagatc gattcgccct tcgtgcgcac cacctatccg
      421 gatgcctggg tttcccgcta cctcctcaac agctatgtga aggtcgatcc gatcgtcaag
      481 cagggcttcg aacgccagct gcccttcgac tggagcgagg tcgaaccgac gccggaggcc
      541 tatgccatgc tggtcgacgc ccagaaacac ggcatcggtg gcaatggcta ctccatcccc
      601 gtcgccgaca aggcgcagcg ccgcgccctg ctgtcgctga atgcccgtat accggccgac
      661 gaatggaccg agctcgtgcg ccgctgccgc aacgagtgga tcgagatcgc ccatctgatc
      721 caccgcaagg ccgtctatga gctgcatggc gaaaacgatc cggtgccggc attgtcgccg
      781 cgcgagatcg agtgtctgca ctggaccgcc ctcggcaagg attacaagga tatttcggtc
      841 atcctgggca tatcagagca taccacacgc gattacctga agaccgcccg cttcaagctc
      901 ggctgcgcca cgatctcggc cgccgcgtcg cgggctgttc aattgcgcat catcaatccc
      961 gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga
     1021 tcgctactag agattaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa
     1081 gagttcgaca aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa
     1141 cgtaaaggtt gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg
     1201 tccccgtact acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg
     1261 cgtatcttcg acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac
     1321 ggtaaagaag ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc
     1381 ggtcagaaag gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct
     1441 cgttactcct tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa
     1501 aaaatgatga tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt
     1561 atcgaacgtg ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac
     1621 ggtggtgttc tggttgaaca gcgtctggct gtttccgctg ctaacgacga aaactacgct
     1681 ctggttgctt aataactctg atagtgctag tgtagatctc tactagagaa aaaaaaaccc
     1741 cgcccctgac agggcggggt ttttttt
//