LOCUS       BBa_K1375018            2660 bp    DNA     linear       17-May-2024
DEFINITION  Prhl/las +RBS+GFP+DT+Pcon+RBS+LasR+RhlR+DT
ACCESSION   BBa_K1375018
VERSION     BBa_K1375018.1  
FEATURES             Location/Qualifiers
     RBS             64..75
                     /label=b0034
     promoter        1..75
                     /label=BBa_K1375024
     CDS             82..801
                     /label=GFP protein
     CDS             82..801
                     /label=BBa_E0040
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     misc_feature    82..938
                     /label=BBa_I13401
     promoter        947..981
                     /label=BBa_J23100
     misc_feature    994..997
                     /label=conserved
     RBS             990..1001
                     /label=BBa_B0034
     CDS             1008..1724
                     /label=lasR
     misc_feature    1725..1757
                     /label=LVA
     misc_feature    1487..1487
                     /label=G
     misc_feature    1764..1788
                     /label=Help:Barcodes
     CDS             1008..1763
                     /label=BBa_C0079
     misc_feature    990..1788
                     /label=BBa_S03156
     CDS             1795..2523
                     /label=BBa_C0171
     stem_loop       2543..2586
                     /label=stem_loop
     misc_feature    2532..2611
                     /label=BBa_B0010
     terminator      2532..2611
                     /label=BBa_B0010
     stem_loop       2627..2646
                     /label=T7 TE
     misc_feature    2653..2653
                     /label=stop
     polyA_site      2647..2660
                     /label=polya
     misc_feature    2620..2660
                     /label=BBa_B0012
     terminator      2620..2660
                     /label=BBa_B0012
     terminator      2532..2660
                     /label=BBa_B0015
ORIGIN
        1 tcctgtgaaa tctggcagtt accgatctat ctcatttgct agttataatg tgttcactac
       61 tagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cggctagctc
      961 agtcctaggt acagtgctag ctactagaga aagaggagaa atactagatg gccttggttg
     1021 acggttttct tgagctggaa cgctcaagtg gaaaattgga gtggagcgcc atcctccaga
     1081 agatggcgag cgaccttgga ttctcgaaga tcctgttcgg cctgttgcct aaggacagcc
     1141 aggactacga gaacgccttc atcgtcggca actacccggc cgcctggcgc gagcattacg
     1201 accgggctgg ctacgcgcgg gtcgacccga cggtcagtca ctgtacccag agcgtactgc
     1261 cgattttctg ggaaccgtcc atctaccaga cgcgaaagca gcacgagttc ttcgaggaag
     1321 cctcggccgc cggcctggtg tatgggctga ccatgccgct gcatggtgct cgcggcgaac
     1381 tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc cgaggccaac cgtttcatag
     1441 agtcggtcct gccgaccctg tggatgctca aggactacgc actgcaaagc ggtgccggac
     1501 tggccttcga acatccggtc agcaaaccgg tggttctgac cagccgggag aaggaagtgt
     1561 tgcagtggtg cgccatcggc aagaccagtt gggagatatc ggttatctgc aactgctcgg
     1621 aagccaatgt gaacttccat atgggaaata ttcggcggaa gttcggtgtg acctcccgcc
     1681 gcgtagcggc cattatggcc gttaatttgg gtcttattac tctcgctgca aacgacgaaa
     1741 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagatgagg
     1801 aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca gccgatccac
     1861 gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg cttcgattac
     1921 tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga ggtccatggc
     1981 acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc cgtggatccg
     2041 gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag cctgttcgac
     2101 cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg cgcgaccttg
     2161 ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga ccagcagaac
     2221 atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat cgagttgctg
     2281 acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt ctgcctgagc
     2341 catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg ggaaatcgcc
     2401 atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat ccagaagaag
     2461 ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg tctcatctaa
     2521 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2581 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2641 tgggcctttc tgcgtttata
//