##gff-version 3
##sequence-region BBa_K1375018 1 2660
BBa_K1375018	.	CDS	1795	2523	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1375018	.	promoter	947	981	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1375018	.	terminator	2532	2660	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1375018	.	terminator	2532	2611	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375018	.	stem_loop	2543	2586	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375018	.	engineered_region	2532	2611	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375018	.	terminator	2620	2660	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375018	.	stem_loop	2627	2646	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375018	.	engineered_region	2620	2660	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375018	.	stop_codon	2653	2653	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375018	.	polyA_site	2647	2660	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375018	.	engineered_region	82	938	.	+	0	ID=BBa_I13401;Name=BBa_I13401
BBa_K1375018	.	CDS	82	801	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13401
BBa_K1375018	.	CDS	82	801	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1375018	.	terminator	898	938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13401
BBa_K1375018	.	stem_loop	905	924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375018	.	engineered_region	898	938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375018	.	stop_codon	931	931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375018	.	polyA_site	925	938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375018	.	terminator	810	889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13401
BBa_K1375018	.	stem_loop	821	864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375018	.	engineered_region	810	889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375018	.	engineered_region	990	1788	.	+	0	ID=BBa_S03156;Name=BBa_S03156
BBa_K1375018	.	CDS	1008	1763	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K1375018	.	engineered_tag	1764	1788	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K1375018	.	tag	1725	1757	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K1375018	.	sequence_alteration	1487	1487	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K1375018	.	CDS	1008	1724	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K1375018	.	ribosome_entry_site	990	1001	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K1375018	.	conserved_region	994	997	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375018	.	promoter	1	75	.	+	0	ID=BBa_K1375024;Name=BBa_K1375024
BBa_K1375018	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2415999;Name=b0034;Parent=BBa_K1375024
>BBa_K1375018
tcctgtgaaatctggcagttaccgatctatctcatttgctagttataatgtgttcactactagaaagaggagaaatact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtt
gacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggttt
tcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcg
aagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcct
ggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgat
tttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtat
gggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccg
aggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggact
ggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggc
aagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcgga
agttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacga
aaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagatgaggaatgacggaggcttttt
gctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaa
gtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtcc
atggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacgg
cctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgat
tggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgacc
agcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagct
gaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggacc
gccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaaca
tccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata