LOCUS BBa_K1375022 5377 bp DNA linear 22-Dec-2024 DEFINITION promoter+RBS+araC+ DT+pBAD+RBS+Cinr+RBS+CinI+DT+Plac+RBS+LuxI+DT+Pc on+RBS+LacI ACCESSION BBa_K1375022 VERSION BBa_K1375022.1 FEATURES Location/Qualifiers misc_feature 1..1114 /label=BBa_I13458 promoter 1123..1253 /label=promoter misc_feature 1162..1179 /label=AraI1 misc_feature 1183..1200 /label=AraI2 promoter 1123..1252 /label=BBa_K206000 misc_feature 1..1252 /label=BBa_K1375000 stem_loop 1272..1315 /label=stem_loop misc_feature 1261..1340 /label=BBa_B0010 terminator 1261..1340 /label=BBa_B0010 stem_loop 1356..1375 /label=T7 TE misc_feature 1382..1382 /label=stop polyA_site 1376..1389 /label=polya misc_feature 1349..1389 /label=BBa_B0012 terminator 1349..1389 /label=BBa_B0012 terminator 1261..1389 /label=BBa_B0015 promoter 1398..1528 /label=promoter misc_feature 1437..1454 /label=AraI1 misc_feature 1458..1475 /label=AraI2 promoter 1398..1527 /label=BBa_K206000 misc_feature 1540..1543 /label=conserved RBS 1536..1547 /label=BBa_B0034 CDS 1554..2276 /label=cinR misc_feature 2277..2309 /label=LVA misc_feature 2316..2340 /label=Help:Barcodes CDS 1554..2340 /label=BBa_C0077 misc_feature 2353..2356 /label=conserved RBS 2349..2360 /label=BBa_B0034 CDS 2367..3029 /label=CinI misc_feature 3030..3062 /label=LVA misc_feature 3069..3093 /label=Help:Barcodes CDS 2367..3093 /label=BBa_C0076 promoter 1536..3093 /label=BBa_I761000 stem_loop 3109..3128 /label=T7 TE misc_feature 3135..3135 /label=stop polyA_site 3129..3142 /label=polya misc_feature 3102..3142 /label=BBa_B0012 terminator 3102..3142 /label=BBa_B0012 stem_loop 3163..3185 /label=stem_loop misc_feature 3151..3196 /label=BBa_B0011 terminator 3151..3196 /label=BBa_B0011 terminator 3102..3196 /label=BBa_B0014 promoter 1536..3196 /label=BBa_I761014 CDS 3205..3292 /label=end of LacI coding region (inactive) misc_feature 3205..3404 /label=BBa_R0010 misc_feature 3293..3330 /label=CAP binding site promoter 3341..3346 /label=-35 promoter 3365..3370 /label=-10 misc_feature 3370..3404 /label=LacI binding site misc_feature 3377..3377 /label=start promoter 3205..3404 /label=BBa_R0010 misc_feature 3417..3420 /label=conserved RBS 3413..3424 /label=BBa_B0034 CDS 3431..4015 /label=LuxI CDS 3431..4015 /label=BBa_C0161 misc_feature 3205..4015 /label=BBa_K084012 stem_loop 4035..4078 /label=stem_loop misc_feature 4024..4103 /label=BBa_B0010 terminator 4024..4103 /label=BBa_B0010 stem_loop 4119..4138 /label=T7 TE misc_feature 4145..4145 /label=stop polyA_site 4139..4152 /label=polya misc_feature 4112..4152 /label=BBa_B0012 terminator 4112..4152 /label=BBa_B0012 terminator 4024..4152 /label=BBa_B0015 misc_feature 3205..4152 /label=BBa_K1375011 promoter 4161..4195 /label=BBa_J23100 RBS 4204..4218 /label=RBS-1\Strong RBS 4211..4215 /label=RBS misc_feature 4204..4218 /label=BBa_B0030 RBS 4204..4218 /label=BBa_B0030 misc_feature 4161..4218 /label=BBa_K081005 misc_feature 5314..5352 /label=LVA CDS 4225..5352 /label=lacI-LVA misc_feature 4225..5352 /label=BBa_C0012 misc_feature 5353..5377 /label=Help:Barcodes CDS 4225..5352 /label=BBa_C0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg 1141 gcgtcacact ttgctatgcc atagcaagat agtccataag attagcggat cctacctgac 1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag 1261 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1321 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 1381 gcgtttatat actagagaca ttgattattt gcacggcgtc acactttgct atgccatagc 1441 aagatagtcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt 1501 ttctccatac cgtttttttg ggctagctac tagagaaaga ggagaaatac tagatgattg 1561 agaataccta tagcgaaaag ttcgagtccg cgttcgaaca gatcaaggcg gcggccaacg 1621 tggatgccgc catccgtatt ctccaggcgg aatataacct cgatttcgtc acctaccatc 1681 tcgcccagac gatcgcgagc aagatcgatt cgcccttcgt gcgcaccacc tatccggatg 1741 cctgggtttc ccgctacctc ctcaacagct atgtgaaggt cgatccgatc gtcaagcagg 1801 gcttcgaacg ccagctgccc ttcgactgga gcgaggtcga accgacgccg gaggcctatg 1861 ccatgctggt cgacgcccag aaacacggca tcggtggcaa tggctactcc atccccgtcg 1921 ccgacaaggc gcagcgccgc gccctgctgt cgctgaatgc ccgtataccg gccgacgaat 1981 ggaccgagct cgtgcgccgc tgccgcaacg agtggatcga gatcgcccat ctgatccacc 2041 gcaaggccgt ctatgagctg catggcgaaa acgatccggt gccggcattg tcgccgcgcg 2101 agatcgagtg tctgcactgg accgccctcg gcaaggatta caaggatatt tcggtcatcc 2161 tgggcatatc agagcatacc acacgcgatt acctgaagac cgcccgcttc aagctcggct 2221 gcgccacgat ctcggccgcc gcgtcgcggg ctgttcaatt gcgcatcatc aatcccgctg 2281 caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag tgtagatcgc 2341 tactagagaa agaggagaaa tactagatgt tcgttatcat ccaggctcac gaataccaga 2401 aatacgctgc tgttctggac cagatgttcc gtctgcgtaa aaaagttttc gctgacaccc 2461 tgtgctggga cgttccggtt atcggtccgt acgaacgtga ctcctacgac tccctggctc 2521 cggcttacct ggtttggtgc aacgactccc gtacccgtct gtacggtggt atgcgtctga 2581 tgccgaccac cggtccgacc ctgctgtacg acgttttccg tgaaaccttc ccggacgctg 2641 ctgacctgat cgctccgggt atctgggaag gtacccgtat gtgcatcgac gaagaagcta 2701 tcgctaaaga cttcccggaa atcgacgctg gtcgtgcttt ctccatgatg ctgctggctc 2761 tgtgcgaatg cgctctggac cacggtatcc acaccatgat ctccaactac gaaccgtacc 2821 tgaaacgtgt ttacaaacgt gctggtgctg aagttgaaga actgggtcgt gctgacggtt 2881 acggtaaata cccggtttgc tgcggtgctt tcgaagtttc cgaccgtgtt ctgcgtaaaa 2941 tgcgtgctgc tctgggtctg accctgccgc tgtacgttcg tcacgttccg gctcgttccg 3001 ttgttaccca gttcctggaa atggctgctg ctgctaacga cgaaaactac gctctggttg 3061 cttaataacc ctgatagtgc tagtgtagat ccctactaga gtcacactgg ctcaccttcg 3121 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc 3181 ggctttttta ttattttact agagcaatac gcaaaccgcc tctccccgcg cgttggccga 3241 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 3301 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 3361 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacatactag agaaagagga 3421 gaaatactag atgactataa tgataaaaaa atcggatttt ttggcaattc catcggagga 3481 gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg agtgggactt 3541 agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag aatatattta 3601 tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta caacaggtga 3661 ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc ccaaagatcc 3721 taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga taaataactc 3781 tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg ttagtcaagg 3841 tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa agcgtattaa 3901 agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata ctaaatcggt 3961 tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatt aataatacta 4021 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 4081 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 4141 tctgcgttta tatactagag ttgacggcta gctcagtcct aggtacagtg ctagctacta 4201 gagattaaag aggagaaata ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc 4261 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 4321 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 4381 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 4441 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 4501 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 4561 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 4621 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 4681 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 4741 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 4801 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 4861 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 4921 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 4981 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg 5041 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt aaccaccatc 5101 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 5161 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 5221 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 5281 gcacgacagg tttcccgact ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta 5341 gtagcttaat aactctgata gtgctagtgt agatctc //