##gff-version 3
##sequence-region BBa_K1375022 1 5352
BBa_K1375022	.	promoter	1398	1527	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K1375022	.	operator	1458	1475	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1375022	.	promoter	1398	1528	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1375022	.	operator	1437	1454	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1375022	.	engineered_region	4161	4218	.	+	0	ID=BBa_K081005;Name=BBa_K081005
BBa_K1375022	.	ribosome_entry_site	4204	4218	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K1375022	.	ribosome_entry_site	4211	4215	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1375022	.	engineered_region	4204	4218	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1375022	.	ribosome_entry_site	4204	4218	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1375022	.	promoter	4161	4195	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K1375022	.	engineered_region	1	1252	.	+	0	ID=BBa_K1375000;Name=BBa_K1375000
BBa_K1375022	.	promoter	1123	1252	.	+	0	ID=BBa_K206000;Name=BBa_K206000;Parent=BBa_K1375000
BBa_K1375022	.	operator	1183	1200	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1375022	.	promoter	1123	1253	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1375022	.	operator	1162	1179	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1375022	.	engineered_region	1	1114	.	+	0	ID=BBa_I13458;Name=BBa_I13458;Parent=BBa_K1375000
BBa_K1375022	.	promoter	1536	3196	.	+	0	ID=BBa_I761014;Name=BBa_I761014
BBa_K1375022	.	promoter	1536	3093	.	+	0	ID=BBa_I761000;Name=BBa_I761000;Parent=BBa_I761014
BBa_K1375022	.	CDS	1554	2340	.	+	0	ID=BBa_C0077;Name=BBa_C0077;Parent=BBa_I761000
BBa_K1375022	.	CDS	1554	2276	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_K1375022	.	engineered_tag	2316	2340	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_K1375022	.	tag	2277	2309	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_K1375022	.	CDS	2367	3093	.	+	0	ID=BBa_C0076;Name=BBa_C0076;Parent=BBa_I761000
BBa_K1375022	.	engineered_tag	3069	3093	.	+	0	ID=annotation2214001;Name=Help:Barcodes;Parent=BBa_C0076
BBa_K1375022	.	tag	3030	3062	.	+	0	ID=annotation306586;Name=LVA;Parent=BBa_C0076
BBa_K1375022	.	CDS	2367	3029	.	+	0	ID=annotation301035;Name=CinI;Parent=BBa_C0076
BBa_K1375022	.	ribosome_entry_site	2349	2360	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I761000
BBa_K1375022	.	conserved_region	2353	2356	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375022	.	ribosome_entry_site	1536	1547	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I761000
BBa_K1375022	.	conserved_region	1540	1543	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375022	.	terminator	3102	3196	.	+	0	ID=BBa_B0014;Name=BBa_B0014;Parent=BBa_I761014
BBa_K1375022	.	terminator	3151	3196	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K1375022	.	engineered_region	3151	3196	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K1375022	.	stem_loop	3163	3185	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K1375022	.	terminator	3102	3142	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K1375022	.	stem_loop	3109	3128	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375022	.	stop_codon	3135	3135	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375022	.	polyA_site	3129	3142	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375022	.	engineered_region	3102	3142	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375022	.	engineered_region	3205	4152	.	+	0	ID=BBa_K1375011;Name=BBa_K1375011
BBa_K1375022	.	engineered_region	3205	4015	.	+	0	ID=BBa_K084012;Name=BBa_K084012;Parent=BBa_K1375011
BBa_K1375022	.	promoter	3205	3404	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K084012
BBa_K1375022	.	engineered_region	3205	3404	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1375022	.	CDS	3205	3292	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1375022	.	promoter	3365	3370	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1375022	.	start_codon	3377	3377	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1375022	.	non_covalent_binding_site	3370	3404	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1375022	.	non_covalent_binding_site	3293	3330	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1375022	.	promoter	3341	3346	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1375022	.	CDS	3431	4015	.	+	0	ID=BBa_C0161;Name=BBa_C0161;Parent=BBa_K084012
BBa_K1375022	.	CDS	3431	4015	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_K1375022	.	ribosome_entry_site	3413	3424	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K084012
BBa_K1375022	.	conserved_region	3417	3420	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375022	.	terminator	4024	4152	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1375011
BBa_K1375022	.	terminator	4024	4103	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375022	.	stem_loop	4035	4078	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375022	.	engineered_region	4024	4103	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375022	.	terminator	4112	4152	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375022	.	stem_loop	4119	4138	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375022	.	stop_codon	4145	4145	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375022	.	polyA_site	4139	4152	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375022	.	engineered_region	4112	4152	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375022	.	CDS	4225	5352	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1375022	.	engineered_tag	5353	5377	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1375022	.	engineered_region	4225	5352	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1375022	.	tag	5314	5352	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1375022	.	CDS	4225	5352	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1375022	.	terminator	1261	1389	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1375022	.	terminator	1261	1340	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375022	.	stem_loop	1272	1315	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375022	.	engineered_region	1261	1340	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375022	.	terminator	1349	1389	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375022	.	stem_loop	1356	1375	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375022	.	stop_codon	1382	1382	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375022	.	polyA_site	1376	1389	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375022	.	engineered_region	1349	1389	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1375022
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattag
cggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcac
actttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtt
tctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgattgagaatacctatagcgaaaag
ttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcggaatataacc
tcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacctatccgga
tgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaacgccagctg
cccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacggcatcggtg
gcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtataccggccga
cgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaaggccgtctat
gagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgccctcggca
aggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcccgcttcaa
gctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaaacgacgaa
aactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgt
tcgttatcatccaggctcacgaataccagaaatacgctgctgttctggaccagatgttccgtctgcgtaaaaaagtttt
cgctgacaccctgtgctgggacgttccggttatcggtccgtacgaacgtgactcctacgactccctggctccggcttac
ctggtttggtgcaacgactcccgtacccgtctgtacggtggtatgcgtctgatgccgaccaccggtccgaccctgctgt
acgacgttttccgtgaaaccttcccggacgctgctgacctgatcgctccgggtatctgggaaggtacccgtatgtgcat
cgacgaagaagctatcgctaaagacttcccggaaatcgacgctggtcgtgctttctccatgatgctgctggctctgtgc
gaatgcgctctggaccacggtatccacaccatgatctccaactacgaaccgtacctgaaacgtgtttacaaacgtgctg
gtgctgaagttgaagaactgggtcgtgctgacggttacggtaaatacccggtttgctgcggtgctttcgaagtttccga
ccgtgttctgcgtaaaatgcgtgctgctctgggtctgaccctgccgctgtacgttcgtcacgttccggctcgttccgtt
gttacccagttcctggaaatggctgctgctgctaacgacgaaaactacgctctggttgcttaataaccctgatagtgct
agtgtagatccctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatata
aaaagccagattattaatccggcttttttattattttactagagcaatacgcaaaccgcctctccccgcgcgttggccg
attcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagc
tcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatt
tcacacatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcgg
aggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataa
ccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgc
tggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctccca
aagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaat
tacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaaca
gcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgata
ctaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtaca
gtgctagctactagagattaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagag
tatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaag
tggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgat
tggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactg
ggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgc
aacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgt
tccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactg
ggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtc
tgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgc
catgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcag
atggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacg
ataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgt
ggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaa
accaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggttt
cccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtag
atctc