##gff-version 3
##sequence-region BBa_K1375023 1 1714
BBa_K1375023	.	CDS	849	1714	.	+	0	ID=BBa_K1375004;Name=BBa_K1375004
BBa_K1375023	.	terminator	1586	1714	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1375004
BBa_K1375023	.	terminator	1586	1665	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375023	.	stem_loop	1597	1640	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375023	.	engineered_region	1586	1665	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375023	.	terminator	1674	1714	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375023	.	polyA_site	1701	1714	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375023	.	stem_loop	1681	1700	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375023	.	engineered_region	1674	1714	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375023	.	stop_codon	1707	1707	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375023	.	CDS	849	1577	.	+	0	ID=BBa_C0171;Name=BBa_C0171;Parent=BBa_K1375004
BBa_K1375023	.	engineered_region	44	842	.	+	0	ID=BBa_S03156;Name=BBa_S03156
BBa_K1375023	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K1375023	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375023	.	CDS	62	817	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K1375023	.	engineered_tag	818	842	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K1375023	.	CDS	62	778	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K1375023	.	tag	779	811	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K1375023	.	sequence_alteration	541	541	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K1375023	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1375023
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagatgaggaatgacggaggcttt
ttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaagg
aagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggt
ccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaac
ggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcg
attggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcga
ccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaag
ctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatgga
ccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaa
catccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata