LOCUS       BBa_K1375026            1692 bp    DNA     linear       05-Nov-2024
DEFINITION  Plux+RBS+LuxR+RBS+RhlI+DT
ACCESSION   BBa_K1375026
VERSION     BBa_K1375026.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     misc_feature    1..862
                     /label=BBa_J37019
     misc_feature    875..878
                     /label=conserved
     RBS             871..882
                     /label=BBa_B0034
     CDS             889..1491
                     /label=rhlL
     misc_feature    1492..1524
                     /label=LVA
     misc_feature    1531..1555
                     /label=Help:Barcodes
     CDS             889..1530
                     /label=BBa_C0070
     misc_feature    871..1555
                     /label=BBa_I9026
     stem_loop       1575..1618
                     /label=stem_loop
     misc_feature    1564..1643
                     /label=BBa_B0010
     terminator      1564..1643
                     /label=BBa_B0010
     stem_loop       1659..1678
                     /label=T7 TE
     misc_feature    1685..1685
                     /label=stop
     polyA_site      1679..1692
                     /label=polya
     misc_feature    1652..1692
                     /label=BBa_B0012
     terminator      1652..1692
                     /label=BBa_B0012
     terminator      1564..1692
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat gatcgaactg
      901 ctgtccgaat ccctggaagg tctgtccgct gctatgatcg ctgaactggg tcgttaccgt
      961 caccaggttt tcatcgaaaa actgggttgg gacgttgttt ccacctcccg tgttcgtgac
     1021 caggagttcg accagttcga ccacccgcag acccgttaca tcgttgctat gtcccgtcag
     1081 ggtatctgcg gttgcgctcg tctgctgccg accaccgacg cttacctgct gaaagacgtt
     1141 ttcgcttacc tgtgctccga aaccccgccg tccgacccgt ccgtttggga actgtcccgt
     1201 tacgctgctt ccgctgctga cgacccgcag ctggctatga aaatcttctg gtcctccctc
     1261 cagtgcgctt ggtacctggg tgcttcctcc gttgttgctg ttaccaccac cgctatggaa
     1321 cgttacttcg ttcgtaacgg tgttatcctc cagcgtctgg gtccgccgca gaaagttaaa
     1381 ggtgaaaccc tggttgctat ctccttcccg gcttaccagg aacgtggtct ggaaatgctg
     1441 ctgcgttacc acccggaatg gctccagggt gttccgctgt ccatggctgt tgctgcaaac
     1501 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
     1561 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1621 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1681 tctgcgttta ta
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