##gff-version 3
##sequence-region BBa_K1375030 1 2485
BBa_K1375030	.	engineered_region	1	862	.	+	0	ID=BBa_J37019;Name=BBa_J37019
BBa_K1375030	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37019
BBa_K1375030	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375030	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37019
BBa_K1375030	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1375030	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1375030	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1375030	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1375030	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1375030	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1375030	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J37019
BBa_K1375030	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1375030	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1375030	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1375030	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1375030	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1375030	.	terminator	2357	2485	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1375030	.	terminator	2357	2436	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375030	.	stem_loop	2368	2411	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375030	.	engineered_region	2357	2436	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375030	.	terminator	2445	2485	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375030	.	polyA_site	2472	2485	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375030	.	stem_loop	2452	2471	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375030	.	engineered_region	2445	2485	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375030	.	stop_codon	2478	2478	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375030	.	CDS	1562	2348	.	+	0	ID=BBa_C0077;Name=BBa_C0077
BBa_K1375030	.	CDS	1562	2284	.	+	0	ID=annotation301112;Name=cinR;Parent=BBa_C0077
BBa_K1375030	.	tag	2285	2317	.	+	0	ID=annotation306600;Name=LVA;Parent=BBa_C0077
BBa_K1375030	.	engineered_tag	2324	2348	.	+	0	ID=annotation2214000;Name=Help:Barcodes;Parent=BBa_C0077
BBa_K1375030	.	engineered_region	871	1555	.	+	0	ID=BBa_I9026;Name=BBa_I9026
BBa_K1375030	.	CDS	889	1530	.	+	0	ID=BBa_C0070;Name=BBa_C0070;Parent=BBa_I9026
BBa_K1375030	.	CDS	889	1491	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K1375030	.	engineered_tag	1531	1555	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K1375030	.	tag	1492	1524	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K1375030	.	ribosome_entry_site	871	882	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I9026
BBa_K1375030	.	conserved_region	875	878	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1375030
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gaaagaggagaaatactagatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactg
ggtcgttaccgtcaccaggttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagt
tcgaccagttcgaccacccgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgct
gccgaccaccgacgcttacctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtt
tgggaactgtcccgttacgctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagt
gcgcttggtacctgggtgcttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgt
tatcctccagcgtctgggtccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaa
cgtggtctggaaatgctgctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacg
acgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagatgattgagaatacctata
gcgaaaagttcgagtccgcgttcgaacagatcaaggcggcggccaacgtggatgccgccatccgtattctccaggcgga
atataacctcgatttcgtcacctaccatctcgcccagacgatcgcgagcaagatcgattcgcccttcgtgcgcaccacc
tatccggatgcctgggtttcccgctacctcctcaacagctatgtgaaggtcgatccgatcgtcaagcagggcttcgaac
gccagctgcccttcgactggagcgaggtcgaaccgacgccggaggcctatgccatgctggtcgacgcccagaaacacgg
catcggtggcaatggctactccatccccgtcgccgacaaggcgcagcgccgcgccctgctgtcgctgaatgcccgtata
ccggccgacgaatggaccgagctcgtgcgccgctgccgcaacgagtggatcgagatcgcccatctgatccaccgcaagg
ccgtctatgagctgcatggcgaaaacgatccggtgccggcattgtcgccgcgcgagatcgagtgtctgcactggaccgc
cctcggcaaggattacaaggatatttcggtcatcctgggcatatcagagcataccacacgcgattacctgaagaccgcc
cgcttcaagctcggctgcgccacgatctcggccgccgcgtcgcgggctgttcaattgcgcatcatcaatcccgctgcaa
acgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata