LOCUS       BBa_K1375032            2173 bp    DNA     linear       22-Dec-2024
DEFINITION  Plac+RBS+LuxI+DT+Pcon+RBS+LacI
ACCESSION   BBa_K1375032
VERSION     BBa_K1375032.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..811
                     /label=LuxI
     CDS             227..811
                     /label=BBa_C0161
     misc_feature    1..811
                     /label=BBa_K084012
     stem_loop       831..874
                     /label=stem_loop
     misc_feature    820..899
                     /label=BBa_B0010
     terminator      820..899
                     /label=BBa_B0010
     stem_loop       915..934
                     /label=T7 TE
     misc_feature    941..941
                     /label=stop
     polyA_site      935..948
                     /label=polya
     misc_feature    908..948
                     /label=BBa_B0012
     terminator      908..948
                     /label=BBa_B0012
     terminator      820..948
                     /label=BBa_B0015
     misc_feature    1..948
                     /label=BBa_K1375011
     promoter        957..991
                     /label=BBa_J23100
     RBS             1000..1014
                     /label=RBS-1\Strong
     RBS             1007..1011
                     /label=RBS
     misc_feature    1000..1014
                     /label=BBa_B0030
     RBS             1000..1014
                     /label=BBa_B0030
     misc_feature    957..1014
                     /label=BBa_K081005
     misc_feature    2110..2148
                     /label=LVA
     CDS             1021..2148
                     /label=lacI-LVA
     misc_feature    1021..2148
                     /label=BBa_C0012
     misc_feature    2149..2173
                     /label=Help:Barcodes
     CDS             1021..2148
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaattaata atactagagc caggcatcaa ataaaacgaa
      841 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      901 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga
      961 cggctagctc agtcctaggt acagtgctag ctactagaga ttaaagagga gaaatactag
     1021 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     1081 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     1141 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     1201 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     1261 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     1321 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     1381 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     1441 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     1501 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     1561 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     1621 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     1681 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     1741 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     1801 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     1861 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     1921 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     1981 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     2041 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     2101 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     2161 tagtgtagat ctc
//