##gff-version 3
##sequence-region BBa_K1375032 1 2148
BBa_K1375032	.	CDS	1021	2148	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K1375032	.	engineered_tag	2149	2173	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1375032	.	tag	2110	2148	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1375032	.	engineered_region	1021	2148	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1375032	.	CDS	1021	2148	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1375032	.	engineered_region	1	948	.	+	0	ID=BBa_K1375011;Name=BBa_K1375011
BBa_K1375032	.	terminator	820	948	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1375011
BBa_K1375032	.	terminator	820	899	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1375032	.	engineered_region	820	899	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1375032	.	stem_loop	831	874	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1375032	.	terminator	908	948	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1375032	.	polyA_site	935	948	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1375032	.	engineered_region	908	948	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1375032	.	stop_codon	941	941	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1375032	.	stem_loop	915	934	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1375032	.	engineered_region	1	811	.	+	0	ID=BBa_K084012;Name=BBa_K084012;Parent=BBa_K1375011
BBa_K1375032	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K084012
BBa_K1375032	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1375032	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K084012
BBa_K1375032	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1375032	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1375032	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1375032	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1375032	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1375032	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1375032	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1375032	.	CDS	227	811	.	+	0	ID=BBa_C0161;Name=BBa_C0161;Parent=BBa_K084012
BBa_K1375032	.	CDS	227	811	.	+	0	ID=annotation1891615;Name=LuxI;Parent=BBa_C0161
BBa_K1375032	.	engineered_region	957	1014	.	+	0	ID=BBa_K081005;Name=BBa_K081005
BBa_K1375032	.	promoter	957	991	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_K081005
BBa_K1375032	.	ribosome_entry_site	1000	1014	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081005
BBa_K1375032	.	engineered_region	1000	1014	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1375032	.	ribosome_entry_site	1007	1011	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1375032	.	ribosome_entry_site	1000	1014	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K1375032
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgactataat
gataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaag
caaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatattt
atgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgt
ttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaa
aatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtc
aaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcg
tattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaa
aaagcagtcttaaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggtga
atgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaacca
ggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtg
gcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaa
ttgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcga
agcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccag
gatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaaca
gtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgct
gttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaatt
cagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggca
tcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcg
cgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatc
aaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggca
atcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgtt
ggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctc